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Volume 67, Issue 7

sp. nov., an antibiotic-producing bacterium isolated from a sea harbour Free

Abstract

Three heterotrophic, aerobic, brown-pigmented strains, designated P97, P100 and P104, were isolated from a harbour in the southern North Sea. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates are affiliated to the genus DNA–DNA hybridization of the genome of strain P97 against those of existing type species indicated that P97 represents a novel species within the genus , with T5 as the closest described organism (29.6 % DNA–DNA relatedness) followed by CIP 105210 (26.4 %). DNA–DNA hybridization demonstrated that the three new strains belong to the same species. The new isolates inhibited DSM 14096, and were Gram-stain-negative, catalase- and oxidase-positive, chemo-organoheterotrophic and motile. Growth occurred at pH 6.5–9.5 (optimum 7.0–8.0) and at 4–30 °C (optimum 20–28 °C). The strains required NaCl for growth. The salinity range was 0.5–6.0 % (w/v) NaCl for P97 and P100, and 0.5–5.0 % for P104, lower than values described for and . The optimum NaCl concentration for strains P97 and P104 was 2.0–4.0 %, and for P100 was 2.0–3.0 %. Fatty acids (>1 %) comprised 18 : 1ω7c, 16 : 0, 18 : 1 ω7c 11-methyl, 18 : 0, 12 :1, 18 : 2ω7c,12, 10 : 0 3-OH and 12 : 0 3-OH. Polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, one unknown lipid and one additional unknown lipid in strain P97. The major respiratory quinone was Q10. Based on phylogenetic and phenotypic differences, the strains represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is P97 (=DSM 103148=LMG 29594).

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Keyword(s): barnacles , biofilm , environmental niche , Phaeobacter , Roseobacter and tropodithietic acid
© 2017 IUMS
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2017-07-01
2024-04-20
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Phaeobacter porticola sp. nov., an antibiotic-producing bacterium isolated from a sea harbour
Int J Syst Evol Microbiol 67, 2153 (2017); https://doi.org/10.1099/ijsem.0.001879
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