1887

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Volume 67, Issue 6

gen. nov., sp. nov., a sulfur-inhibited thermoacidophilic archaeon belonging to the order isolated from a terrestrial acidic hot spring Free

Abstract

A novel thermoacidophilic archaeon, strain HS-1, was isolated from the Hakone Ohwaku-dani hot spring in Japan. Cells of strain HS-1 in exponential phase were cocci to irregular cocci with a diameter of 0.8–1.5 µm. The strain grew within a temperature range of 50–70 °C (optimal: 65–70 °C), a pH range of pH 1.4–5.5 (optimal: pH 3.0–3.5) and a NaCl concentration range of 0–2.5 % (w/v). The novel strain grew in aerobic conditions but did not grow anaerobically. Moreover, this strain utilized various complex substrates (beef extract, casamino acids, peptone, tryptone and yeast extract) and sugars (arabinose, xylose, galactose, glucose, maltose, sucrose, raffinose and lactose) as sole carbon sources. No chemolithoautotrophic growth occurred on elemental sulfur, pyrite, KSO, NaSO or FeSO . 7HO; however, growth by the oxidation of hydrogen occurred weakly. The core lipids were calditoglycerocaldarchaeol (CGTE) and caldarchaeol (DGTE). The DNA G+C content of the strain was 52.0 mol%, which was remarkably higher than those of known species of the order (31–46.2 %). The growth of the strain was significantly inhibited in the presence of elemental sulfur. Analyses of 16S rRNA and 23S rRNA gene sequences showed that HS-1 belonged to the order ; however, it was distantly related to all known species of the order (less than 89 % sequence similarity). On the basis of these results, we propose the novel genus, , in the order (in the family ). The type species of the genus is sp. nov., and the type strain of the species is HS-1 (=JCM 31740=InaCC Ar79).

  • Received:
  • Accepted:
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Keyword(s): acidophiles , archaea , Sulfolobales , sulfur-inhibited , terrestrial acidic hot springs and thermophiles
© 2017 IUMS
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2017-06-01
2024-04-25
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Sulfodiicoccus acidiphilus gen. nov., sp. nov., a sulfur-inhibited thermoacidophilic archaeon belonging to the order Sulfolobales isolated from a terrestrial acidic hot spring
Int J Syst Evol Microbiol 67, 1880 (2017); https://doi.org/10.1099/ijsem.0.001881
/content/journal/ijsem/10.1099/ijsem.0.001881
/content/journal/ijsem/10.1099/ijsem.0.001881
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