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f Photobacterium alginatilyticum sp. nov., a marine bacterium isolated from bottom seawater

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  • Authors: Xiaolei Wang1​ ,  Yanan Wang1​ ,  Xiaoting Yang1​ ,  Hao Sun1​ ,  Bei Li1​ , Xiao–Hua Zhang1​,2​,3​
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    • 1​ 1​College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China 2​ 2​Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China 3​ 3​Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China
  • *Correspondence: Xiao–Hua Zhang, [email protected]
  • First Published Online: 14 June 2017, International Journal of Systematic and Evolutionary Microbiology 67: 1912-1917, doi: 10.1099/ijsem.0.001886
  • Subject: New Taxa - Proteobacteria
  • Received:
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Photobacterium alginatilyticum sp. nov., a marine bacterium isolated from bottom seawater, Page 1 of 1

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  • A Gram-straining-negative, facultatively aerobic, rod-shaped strain, motile by a polar flagellum and designated P03D4, was isolated from the bottom seawater of the East China Sea. Growth occurred at 10–50 °C (optimum 32 °C), pH 5.0–10.0 (optimum pH 6.0) and in the presence of 1–7 % (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequence placed P03D4 within the genus Photobacterium of the family Vibrionaceae in the class Gammaproteobacteria , and revealed that strain P03D4 was most closely related to Photobacterium frigidiphilum SL13 with 96.9 % sequence similarity and had sequence similarities with other species of the genus Photobacterium in the range 94.6–96.9 %. The dominant fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The polar lipids of strain P03D4 comprised phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and one unknown lipid. The major respiratory quinone was ubiquinone-8 (Q-8). The DNA G+C content of strain P03D4 was 44.3 mol%. On the basis of the evidence from this polyphasic study, strain P03D4 is proposed as representing a novel species of the genus Photobacterium , for which the name Photobacterium alginatilyticum sp. nov. is proposed. The type strain is P03D4 (=KCTC 52365=MCCC 1K03200=CGMCC 1.15764).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of P03D4 is KU315478.

  • Two supplementary figures and one supplementary table are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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