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f Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata

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  • Authors: Munusamy Madhaiyan1​,2​ ,  Selvaraj Poonguzhali1​,2​ ,  Venkatakrishnan Sivaraj Saravanan1​,3​ ,  Veeramuthu Duraipandiyan4​,5​ ,  Naif Abdullah Al-Dhabi4​ ,  Soon-Wo Kwon6​ , William B. Whitman7​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India 2​ 2​Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore 3​ 3​Department of Microbiology, Indira Gandhi College of Arts and Science, Kathirkamam 605009, Pondicherry, India 4​ 4​Addiriyah Research Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia 5​ 5​Entomology Research Institute, Loyola College, Chennai 600034, India 6​ 6​Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea 7​ 7​Department of Microbiology, University of Georgia, 527 Biological Sciences Building, Athens, GA 30602-2605, USA
  • *Correspondence: Munusamy Madhaiyan, [email protected]
  • First Published Online: 03 July 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2127-2133, doi: 10.1099/ijsem.0.001901
  • Subject: New Taxa - Firmicutes and Related Organisms
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Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata, Page 1 of 1

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  • A novel xylanolytic and cellulolytic strain, BL9, was isolated from leaves of the Bamboo plants maintained at the Tamil Nadu Agricultural University Campus, Coimbatore, India. On the basis of the results of 16S rRNA gene analysis, it was determined to be phylogenetically close to the type strains of Paenibacillus amylolyticus NRRL NRS-290 (98.3 %), Paenibacillus barcinonensis BP-23 (98.1 %), Paenibacillus tundrae A10b (98.0 %) and Paenibacillus xylanexedens B22a (97.6 %). The strain stained Gram variable and was aerobic, motile and catalase- and oxidase-positive, with rod-shaped cells. Based upon the genome sequence, the average nucleotide identity with the related species ranged from 66 % to 72 %, and the digital DNA–DNA hybridization value ranged from 13 % to 27 %. The DNA G+C content was 45.6 mol%, meso-diaminopimelic acid was identified as the predominant component of the cell wall, and MK-7 was the only menaquinone in cell membranes. The whole-cell fatty acid profile included C16 : 0, iso-C14 : 0, anteiso-C15 : 0 and iso-C16 : 0. The polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphtidylglycerol. On the basis of these polyphasic taxonomic traits, BL9 represents a novel species of the genus Paenibacillus , for which the name Paenibacillus polysaccharolyticus sp. nov. is proposed. The type strain is BL9 (=NBRC 105191=ICMP 17623).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and genome sequence of strain BL9 is EU912452 and FMVM01000000 respectively.

  • Five supplementary figures and two supplementary tables are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2019-05-27
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