1887

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Volume 67, Issue 6

sp. nov., isolated from a saline soil by dilution-to-extinction cultivation Free

Abstract

We isolated a Gram-stain-negative, aerobic bacterial strain, 912, from a soil sample taken from Rambla Salada (Murcia), south-eastern Spain, by using the dilution-to-extinction method. Cells of the strain were motile with a polar flagellum, short rod-shaped, catalase- and oxidase-positive and grew at NaCl concentrations within the range 0–5 % (w/v) (optimum 3 %, w/v), at 4–32 °C (optimum 30 °C) and at pH 6–9 (optimum pH 7); bacteriochlorophyll was produced. Analysis of the 16S rRNA gene sequence indicated that this strain belonged to the genus in the class . Its closest relatives were EY 4220, KR-99 and PE 4-5, to which the strain showed 16S rRNA gene sequence similarity values of 95.9, 95.8 and 95.1 %, respectively. The DNA G+C content was 63 mol%. The major fatty acids of strain 912 were Cω7c/C ω6, Cω7c/C ω6, C and Cω6. The predominant isoprenoid quinone was ubiquinone 10 (Q-10). The polar lipids contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphoglycolipid, one unidentified phospholipid and two unidentified polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus , for which the name sp. nov. is proposed. Strain 912 (=CECT 9186=LMG 29921) is the type strain.

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Keyword(s): Blastomonas quesadae sp. nov. , chemotaxonomy , phylogenetic and polyphasic taxonomy
© 2017 IUMS
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2017-06-01
2024-04-18
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Blastomonas quesadae sp. nov., isolated from a saline soil by dilution-to-extinction cultivation
Int J Syst Evol Microbiol 67, 2001 (2017); https://doi.org/10.1099/ijsem.0.001902
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