1887

Abstract

A novel strain designated 11G32 was isolated from an agricultural soil cultivated with Chinese cabbage in Korea. The cells were Gram-stain-negative, aerobic, non-motile and rod-shaped. The strain grew at 15–28 °C (optimum, 20 °C), pH 5.0–7.0 (optimum, pH 5.0–6.0) and without NaCl. Phylogenetically, the strain was found to be closely related to members of the genus and showed 16S rRNA gene sequence similarities of 97.18, 96.76 and 95.99 % with Wo-34, 521 and KIS14-15, respectively. The major fatty acids were C cyclo ω8, C, summed feature 8 (Cω7 and/or Cω6) and 11-methyl Cω7. The predominant ubiquinone was Q-10. The polar lipids profile revealed the presence of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unidentified aminolipids, unidentified phospholipids and unidentified lipids. On the basis of data presented, strain 11G32 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 11G32 (=KACC 18486=NBRC 111476).

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2017-06-01
2024-03-29
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References

  1. Pagnier I, Raoult D, La Scola B. Isolation and characterization of Reyranella massiliensis gen. nov., sp. nov. from freshwater samples by using an amoeba co-culture procedure. Int J Syst Evol Microbiol 2011; 61:2151–2154 [View Article][PubMed]
    [Google Scholar]
  2. Kim S-J, Ahn J-H, Lee T-H, Weon H-Y, Hong S-B et al. Reyranella soli sp. nov., isolated from forest soil, and emended description of the genus Reyranella Pagnier et al. 2011. Int J Syst Evol Microbiol 2013; 63:3164–3167 [View Article]
    [Google Scholar]
  3. Pagnier I, Croce O, Robert C, Raoult D, La Scola B. Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae. J Bacteriol 2012; 194:5698 [View Article][PubMed]
    [Google Scholar]
  4. Lee JC, Whang KS. Reyranella graminifolii sp. nov., isolated from bamboo (Phyllostachys bambusoides) litter. Int J Syst Evol Microbiol 2014; 64:2503–2507 [View Article][PubMed]
    [Google Scholar]
  5. Kim DU, Ka JO. Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 2014; 64:1024–1029 [View Article][PubMed]
    [Google Scholar]
  6. Yoon SH, Sm H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017: in press
    [Google Scholar]
  7. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  8. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York, NY: Academic Press; 1969 pp. 21–132 [CrossRef]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  10. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  13. Breznak JA, Costilow RN. Physicochemical factors in growth. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society For Microbiology; 2007 pp. 309–329
    [Google Scholar]
  14. Smibert R, Krieg N. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington,DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  15. Ten LN, Xu JL, Jin FX, Im WT, Oh HM et al. Spirosoma panaciterrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 2009; 59:331–335 [View Article][PubMed]
    [Google Scholar]
  16. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
  17. Komagata K, Suzuki K-I. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
    [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  19. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 121–161
    [Google Scholar]
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