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Volume 67, Issue 7

gen. nov., sp. nov., isolated from coastal seawater Free

Abstract

A taxonomic study was performed on strain SYSU D3-2, isolated from coastal seawater near the estuary of Pearl River in southern China. The strain was observed to be Gram-reaction-negative, non-motile and non-spore-forming. Cells were found to be of coccobacilli shape. Chemotaxonomic analysis of the plasma membrane revealed ubiquinone-8 as the respiratory quinone, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified phospholipid as the polar lipids, and anteiso-C, C and anteiso-C as the major fatty acids (>10 % of total fatty acids). Comparison of 16S rRNA gene sequences showed that strain SYSU D3-2 shared maximum similarities with 51 (92.3 %) and UST040201-002 (90.6 %), while sharing 85.8–90.0 % similarity with species of the genera and . Phylogenetic dendrograms based on the 16S rRNA gene sequences showed that the strain clustered within the family , but formed a separate lineage closely linked to 51 and UST040201-002. Based on the findings of the polyphasic taxonomic study, strain SYSU D3-2 is proposed to be recognized as a representative of a novel species of a new genus within the order , with the name gen. nov., sp. nov. The type strain of the type species is SYSU D3-2 (=NBRC 112441=DSM 101832=KCTC 52386=CGMCC 1.15758).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Cysteiniphilum litorale gen. nov., sp. nov. and polyphasic taxonomy
© 2017 IUMS
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2017-07-01
2024-04-18
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References

  1. Qu P, Deng X, Zhang J, Chen J, Zhang J et al. Identification and characterization of the Francisella sp. strain 08HL01032 isolated in air condition systems. Wei sheng wu xue bao=Acta Microbiol Sinica 2009; 49:1003–1010
     [Google Scholar]
  2. Qu PH, Chen SY, Scholz HC, Busse HJ, Gu Q et al. Francisella guangzhouensis sp. nov., isolated from air-conditioning systems. Int J Syst Evol Microbiol 2013; 63:3628–3635 [View Article][PubMed]
     [Google Scholar]
  3. Qu PH, Li Y, Salam N, Chen SY, Liu L et al. Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems and transfer of Francisella guangzhouensis Qu, et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov. Int J Syst Evol Microbiol 2016:4832–4838
     [Google Scholar]
  4. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
     [Google Scholar]
  5. Liu L, Salam N, Jiao JY, Jiang HC, Zhou EM et al. Diversity of culturable thermophilic actinobacteria in hot springs in Tengchong, China and studies of their biosynthetic gene profiles. Microb Ecol 2016; 72:150–162 [View Article][PubMed]
     [Google Scholar]
  6. Yoon SH, Sm H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017: in press
    [Google Scholar]
  7. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  8. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  10. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  11. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  12. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  13. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  15. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by High-Performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  16. Leifson E. Atlas of Bacterial Flagellation London: Academic Press; 1960 [CrossRef]
     [Google Scholar]
  17. Liu L, Zhou EM, Jiao JY, Manikprabhu D, Ming H et al. Hymenobacter mucosus sp. nov., isolated from a karst cave soil sample. Int J Syst Evol Microbiol 2015; 65:4121–4127 [View Article][PubMed]
     [Google Scholar]
  18. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993[PubMed]
     [Google Scholar]
  19. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
     [Google Scholar]
  20. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703–704 [View Article][PubMed]
     [Google Scholar]
  21. Aslanzadeh J. Biochemical profile-based microbial identification systems [M]. In Tang YW, Stratton CW et al. Advanced Techniques in Diagnostic Microbiology New York: Springer; 2013 pp. 87–121 [CrossRef]
     [Google Scholar]
  22. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  23. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
     [Google Scholar]
  24. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
     [Google Scholar]
  25. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
     [Google Scholar]
  26. Lau KW, Ren J, Fung MC, Woo PC, Yuen KY et al. Fangia hongkongensis gen. nov., sp. nov., a novel gammaproteobacterium of the order Thiotrichales isolated from coastal seawater of Hong Kong. Int J Syst Evol Microbiol 2007; 57:2665–2669 [View Article][PubMed]
     [Google Scholar]
  27. Sjödin A, Svensson K, Ohrman C, Ahlinder J, Lindgren P et al. Genome characterisation of the genus Francisella reveals insight into similar evolutionary paths in pathogens of mammals and fish. BMC Genomics 2012; 13:268 [View Article][PubMed]
     [Google Scholar]
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Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater
Int J Syst Evol Microbiol 67, 2178 (2017); https://doi.org/10.1099/ijsem.0.001917
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