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f Hymenobacter tenuis sp. nov., isolated from wastewater of an acidic water neutralization facility

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  • Authors: Joo Won Kang1​ ,  Ji Hee Lee1​ ,  Han Na Choe2​ , Chi Nam Seong1​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2​ 2​Biological Resource Center, KRIBB, Jeongeup 56212, Republic of Korea
  • *Correspondence: Chi Nam Seong, [email protected]
  • First Published Online: 08 July 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2171-2177, doi: 10.1099/ijsem.0.001918
  • Subject: New Taxa - Bacteroidetes
  • Received:
  • Accepted:
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  • A non-motile, red–pink-coloured, rod-shaped bacterium, designated strain POB6, was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, C16 : 1ω5c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The strain contained menaquinone MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The DNA G+C content was 61 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain POB6 forms a distinct evolutionary lineage within the radiation enclosing the members of the genus Hymenobacter , sharing the highest similarity with Hymenobacter rigui WPCP131 (97.5 %) followed by Hymenobacter xinjiangensis X2-1g (97.2 %), Hymenobacter perfusus A1-12 (97.2 %) and Hymenobacter gelipurpurascens Txg-1 (97.1 %). A number of phenotypic characteristics distinguished strain POB6 from related members of the genus Hymenobacter . On the basis of the evidence presented in this study, a novel species, Hymenobacter tenuis sp. nov., is proposed, with POB6 (=KCTC 52271=JCM 31659) as the type strain.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain POB6 is KY196418.

  • Three supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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