%0 Journal Article %A Subhash, Y. %A Kim, Hye-ji %A Lee, Sang-Seob %T Aneurinibacillus sediminis sp. nov., isolated from lagoon sediments %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 8 %P 2544-2548 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001963 %K Paenibacillaceae %K Aneurinibacillus sediminis %K Lagoon sediments %I Microbiology Society, %X Two bacterial strains (1-10M-8-7-50T and 1M) were isolated from lagoon sediments. The strains were obligately aerobic, Gram-stain-positive, spore-forming, rod-shaped and motile. Both strains were able to grow at 25–60 ˚C (optimum 40–42 ˚C), at pH 6.0–8.8 (optimum pH 7.0–7.5) and with 0–2.0 % (w/v) (optimum 0–0.8 %) NaCl. The predominant cellular fatty acids were iso-C15 : 0 and iso-C15 : 0 2-OH, and the cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, glycine and alanine. Phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phosphoglycolipids and three unidentified lipids were present as polar lipids. Genomic DNA G+C content was 41.9 mol%. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus Aneurinibacillus within the family Paenibacillaceae of the class Bacilli . Both strains had a sequence similarity of 97.6 % with Aneurinibacillus migulanus B0270T, 97.6 % with Aneurinibacillus aneurinilyticus ATCC 12856T, 97.5 % with ‘ Aneurinibacillus humi ' U33 and <97 % with other members of the genus Aneurinibacillus . The DNA–DNA reassociation value between strain 1-10M-8-7-50T and 1M was >90 %, while strains 1-10M-8-7-50T and 1M were only 35.1±1, 29.8±1 and 24.5±1 % related to A. migulanus KACC 18173T, A. aneurinilyticus KACC 18174T and ‘ A. humi ' U33, respectively. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain 1-10M-8-7-50T as a representative of a novel species in the genus Aneurinibacillus , for which the name Aneurinibacillus sediminis sp. nov. is proposed. The type strain is 1-10M-8-7-50T (=KEMB 563-460T=JCM 31819T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001963