1887

Abstract

Strain AFT2 was isolated from a mural painting sample from a . 1500-year-old tomb located in Shanxi Province, China. The isolate was a Gram-stain-positive, non-motile, non-spore-forming, aerobic and oval to short-rod-shaped bacterium that formed white-pigmented colonies. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain AFT2 was most closely (97.01 %) correlated and formed a monophyletic clade with WS4616 (=DSM 24323). The G+C content of the genomic DNA was 71.97 mol%, and the strain showed 37.27 % DNA–DNA relatedness to DSM 24323. The major cellular fatty acid was anteiso-C (55.32 %), and MK-9(H) was the only respiratory quinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and five unknown glycolipids. -Diaminopimelic acid was detected in the cell-wall peptidoglycan (type A3), and the whole-cell sugars consisted of ribose, mannose, arabinose and galactose. On the basis of its phenotypic and phylogenetic characteristics, it is proposed that strain AFT2 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AFT2 (=CCTCC AB 2015428=DSM 103164).

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2017-08-01
2024-03-28
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