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Volume 67, Issue 7

gen. nov., sp. nov., a thermophilic bacterium of the family isolated from the crop of a laying hen ( f. ) Free

Abstract

Bacteria with potential probiotic applications are not yet sufficiently explored, even for animals with economic importance. Therefore, we decided to isolate and identify representatives of the family , which inhabit the crop of laying hens. During the study, a fructose-6-phosphate phosphoketolase-positive strain, RP51, with a regular/slightly irregular and sometimes an S-shaped slightly curved rod-like shape, was isolated from the crop of a 13 -month-old Hisex Brown hybrid laying hen. The best growth of the Gram-stain-positive bacterium, which was isolated using -selective mTPY agar, was found out to be under strictly anaerobic conditions, however an ability to grow under microaerophilic and aerobic conditions was also observed. Sequencing of the almost complete 16S rRNA gene (1444 bp) showed CCUG 31649 and BLAPIII/AGV to be the most closely related species with similarities of 93.4 and 93.1 %, respectively. Lower sequence similarities were determined with other scardovial genera and other representatives of the genus Taxonomic relationships with and other members of the family were also demonstrated, based on the sequences of , , and gene fragments. Low sequence similarities of phylogenetic markers to related scardovial genera and bifidobacteria along with unique features of the bacterial strain investigated within the family including the lowest DNA G+C value (44.3 mol%), a unique spectrum of cellular fatty acids and polar lipids, cellular morphology, the wide temperature range for growth (15–49 °C) and habitat) clearly indicate that strain RP51 is a representative of a novel genus within the family for which the name gen. nov., sp. nov. (RP51=DSM 100235=LMG 28778=CCM 8606) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bifidobacteriaceae , crop , laying hen and scardovia
© 2017 IUMS
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2017-07-01
2024-04-25
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Galliscardovia ingluviei gen. nov., sp. nov., a thermophilic bacterium of the family Bifidobacteriaceae isolated from the crop of a laying hen (Gallus gallus f. domestica)
Int J Syst Evol Microbiol 67, 2403 (2017); https://doi.org/10.1099/ijsem.0.001972
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