1887

Abstract

Three strains of a Gram-stain-negative, moderately halophilic bacterium, designated strain KHM44, KHM29 and HHM4, were isolated from the sediment of a grey saltern located on Sinui island at Shinan, Korea. The isolates were aerobic, non-motile rods and grew at 15–50 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 8.0) and at salinities of 3–25 % (w/v) NaCl (optimum, 10 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the major fatty acids were iso-C, Cω7 and/or iso-C 2-OH, iso-Cω9 and anteiso-C. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and glycolipid. The DNA G+C contents were 48.5–48.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains KHM44, KHM29 and HHM4 belonged to the genus in the family , with sequence similarities of 95.1–97.2 % to members of this genus. Levels of DNA–DNA relatedness between strain KHM44 and the type strains of the other species of the genus ranged from 35.4 to 48.0 %. On the basis of polyphasic analysis from this study, strains KHM44, KHM29 and HHM4 are considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is KHM44 (=KACC 19060=NBRC 112531).

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2017-08-01
2024-03-28
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