1887

Abstract

A Gram-reaction-negative, aerobic, oxidase- and catalase-positive, yellow-pigmented, non-flagellated, rod-shaped bacterium, designed strain SM1501, was isolated from surface seawater of the South China Sea. SM1501 grew at 7–42 °C and with 0–11 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that SM1501 represented a member of the genus , sharing the highest 16S rRNA gene sequence similarity (97.4 %) with and 94.2–96.5 % 16S rRNA gene sequence similarities to other species of the genus with validly published names. The average nucleotide identity (ANI) value and DNA–DNA hybridization value between SM1501 and were only 74.6 and 20.0 %, respectively. The predominant cellular fatty acids of SM1501 were Cω6, Cω7 and summed feature 3 (Cω7 and/or iso-C 2-OH). The major polar lipids of the strain were phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, diphosphatidylglycerol and phosphatidylcholine and the main respiratory quinone of was Q-10. Polyphasic data presented in this paper support the notion that SM1501 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is SM1501 (=KCTC 42669=CCTCC AB 2015396).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001991
2017-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2459.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001991&mimeType=html&fmt=ahah

References

  1. Shiba T, Simidu U. Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 1982; 32:211–217 [View Article]
    [Google Scholar]
  2. Yoon JH, Oh TK, Park YH. Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 2005; 55:71–75 [View Article][PubMed]
    [Google Scholar]
  3. Lee YS, Lee DH, Kahng HY, Kim EM, Jung JS. Erythrobacter gangjinensis sp. nov., a marine bacterium isolated from seawater. Int J Syst Evol Microbiol 2010; 60:1413–1417 [View Article][PubMed]
    [Google Scholar]
  4. Xu M, Xin Y, Yu Y, Zhang J, Zhou Y et al. Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 2010; 60:2215–2220 [View Article][PubMed]
    [Google Scholar]
  5. Jung YT, Park S, Oh TK, Yoon JH. Erythrobacter marinus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62:2050–2055 [View Article][PubMed]
    [Google Scholar]
  6. Jung YT, Park S, Lee JS, Yoon JH. Erythrobacter lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:4184–4190 [View Article][PubMed]
    [Google Scholar]
  7. Tonon LAC, Moreira APB, Thompson F. The family Erythrobacteraceae . In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. (editors) The Prokaryotes: Alphaproteobacteria and Betaproteobacteria Berlin, Heidelberg: Springer Berlin Heidelberg; 2014 pp. 213–235 [CrossRef]
    [Google Scholar]
  8. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  14. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  15. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  16. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  17. Wayne L, Brenner D, Colwell R, Grimont P, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
    [Google Scholar]
  18. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
    [Google Scholar]
  19. Collins MD, Jones D. Lipids in the classification and identification of Coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
    [Google Scholar]
  20. Subhash Y, Tushar L, Sasikala Ch, Ramana ChV. Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 2013; 63:4524–4532 [View Article][PubMed]
    [Google Scholar]
  21. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 21–41
    [Google Scholar]
  22. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  23. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  24. Martens T, Heidorn T, Pukall R, Simon M, Tindall BJ et al. Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . Int J Syst Evol Microbiol 2006; 56:1293–1304 [View Article][PubMed]
    [Google Scholar]
  25. Lei X, Zhang H, Chen Y, Li Y, Chen Z et al. Erythrobacter luteus sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2015; 65:2472–2478 [View Article][PubMed]
    [Google Scholar]
  26. Zhuang L, Liu Y, Wang L, Wang W, Shao Z. Erythrobacter atlanticus sp. nov., a bacterium from ocean sediment able to degrade polycyclic aromatic hydrocarbons. Int J Syst Evol Microbiol 2015; 65:3714–3719 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001991
Loading
/content/journal/ijsem/10.1099/ijsem.0.001991
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error