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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., with the ability to degrade aliphatic hydrocarbons, isolated from oil-contaminated soil Free

Abstract

A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped bacterium, designated K-1-16, that is capable of degrading aliphatic hydrocarbons was isolated from oil-contaminated soil at Biratnagar, Morang, Nepal. It was able to grow at 15–45 °C, at pH 5.5–9.5 and with 0–5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-1-16 belongs to the genus and is closely related to CP173-2 (98.6 % similarity), DS-4 (97.9 %), MA-olki (97.9 %), MA101b (97.8 %) and 10-1-84 (96.6 %). The predominant respiratory quinone was ubiquinone Q-10 and the major polyamine was homospermidine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine and sphingoglycolipid. The predominant fatty acids of strain K-1-16 were summed feature 8 (Cω7 and/or Cω6), C, summed feature 3 (Cω7 and/or Cω6), Cω7 11-methyl and C 2-OH. The genomic DNA G+C content was 64.8 mol%. Levels of DNA–DNA relatedness between strain K-1-16 and DSM 17494, KACC 17420, KCCM 41909 and KCCM 41908 were 49.7, 41.3, 43.7 and 36.7 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain K-1-16 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K-1-16 (=KEMB 9005-450=KACC 19002=JCM 31674).

  • Received:
  • Accepted:
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Keyword(s): aliphatic hydrocarbons , microbial degradation , oil-contaminated soil , Proteobacteria and Sphingomonas olei sp. nov.
© 2017 IUMS
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2017-08-01
2024-04-19
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Sphingomonas olei sp. nov., with the ability to degrade aliphatic hydrocarbons, isolated from oil-contaminated soil
Int J Syst Evol Microbiol 67, 2731 (2017); https://doi.org/10.1099/ijsem.0.002010
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