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- Volume 67, Issue 8
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f Bacillus praedii sp. nov., isolated from purplish paddy soil
- Authors: Bo Liu1,† , Guo-Hong Liu1,† , Cetin Sengonca2 , Peter Schumann3 , Jie-Ping Wang1 , Yu-Jing Zhu1 , Qin-Ying Liu1,4 , Ming-Kuang Wang5
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- VIEW AFFILIATIONS
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1 1Agricultural Bio-resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China 2 2Institute of Crop Sciences and Resource Conservation (INRES), University of Bonn, Meckenheimer Allee 166A D-53115 Bonn, Germany 3 3Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße7B, 38124, Braunschweig, Germany 4 4College of Life Science, Fujian Normal University, Fuzhou, Fujian 350117, PR China 5 5Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, ROC
- *Correspondence: Bo Liu, [email protected]
- First Published Online: 18 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2823-2828, doi: 10.1099/ijsem.0.002030
- Subject: New Taxa - Firmicutes and Related Organisms
- Received:
- Accepted:
- Cover date:




Bacillus praedii sp. nov., isolated from purplish paddy soil, Page 1 of 1
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A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-25547T, was isolated from the purplish paddy soil collected from Linshan Township, Yanting Prefecture of Sichuan Province in PR China (31° 16′ N 105° 27′ E). Growth was achieved aerobically at temperatures between 15 and 40 °C (optimum 30 °C), with between 0 and 10.0 % NaCl (w/v) (optimum 4 %) and in the range of pH 5.0–12.0 (optimum pH 9.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and the main isoprenoid quinone was MK-7. The major fatty acids were iso-C15 : 0 (55.4 %), anteiso-C15 : 0 (22.2 %), iso-C16 : 0 (5.1 %) and iso-C14 : 0 (6.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25547T was a member of the genus Bacillus and was most closely related to Bacillus horneckiae DSM 23495T (97.7 % similarity), Bacillus eiseniae A1-2T (97.5 %), Bacillus mesophilum IITR-54T (97.2 %) and Bacillus kochii WCC 4582T (97.0 %). The average nucleotide identity value between strain FJAT-25547T and the type strain of the most closely related species, B. horneckiae DSM 23495T, was 77.7 %, less than the proposed cut-off value of 96.0 % for differentiating species within the genus. The in silico DNA–DNA hybridization value of strain FJAT-25547T with the most closely related species was 22.7 %, <70 %, again indicating they belong to different taxa. The DNA G+C content of strain FJAT-25547T was 39.1 mol%. This taxono-genomics study revealed that strain FJAT-25547T represents a novel species of the genus Bacillus for which the name Bacillus praedii sp. nov. (type strain FJAT-25547T=CCTCC AB 2015208T=DSM 101002T) is proposed.
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of strain FJAT-25547T are KY582959 and LMBX00000000, respectively.
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Three supplementary tables and four supplementary figures are available with the online Supplementary Material.
© 2017 IUMS | Published by the Microbiology Society
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