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f Bacillus praedii sp. nov., isolated from purplish paddy soil

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  • Authors: Bo Liu1​,† ,  Guo-Hong Liu1​,† ,  Cetin Sengonca2​ ,  Peter Schumann3​ ,  Jie-Ping Wang1​ ,  Yu-Jing Zhu1​ ,  Qin-Ying Liu1​,4​ , Ming-Kuang Wang5​
    • VIEW AFFILIATIONS
    • 1​ 1​Agricultural Bio-resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China 2​ 2​Institute of Crop Sciences and Resource Conservation (INRES), University of Bonn, Meckenheimer Allee 166A D-53115 Bonn, Germany 3​ 3​Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße7B, 38124, Braunschweig, Germany 4​ 4​College of Life Science, Fujian Normal University, Fuzhou, Fujian 350117, PR China 5​ 5​Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, ROC
  • *Correspondence: Bo Liu, [email protected]
  • First Published Online: 18 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 2823-2828, doi: 10.1099/ijsem.0.002030
  • Subject: New Taxa - Firmicutes and Related Organisms
  • Received:
  • Accepted:
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  • A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-25547, was isolated from the purplish paddy soil collected from Linshan Township, Yanting Prefecture of Sichuan Province in PR China (31° 16′ N 105° 27′ E). Growth was achieved aerobically at temperatures between 15 and 40 °C (optimum 30 °C), with between 0 and 10.0 % NaCl (w/v) (optimum 4 %) and in the range of pH 5.0–12.0 (optimum pH 9.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and the main isoprenoid quinone was MK-7. The major fatty acids were iso-C15 : 0 (55.4 %), anteiso-C15 : 0 (22.2 %), iso-C16 : 0 (5.1 %) and iso-C14 : 0 (6.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25547 was a member of the genus Bacillus and was most closely related to Bacillus horneckiae DSM 23495 (97.7 % similarity), Bacillus eiseniae A1-2 (97.5 %), Bacillus mesophilum IITR-54 (97.2 %) and Bacillus kochii WCC 4582 (97.0 %). The average nucleotide identity value between strain FJAT-25547 and the type strain of the most closely related species, B. horneckiae DSM 23495, was 77.7 %, less than the proposed cut-off value of 96.0 % for differentiating species within the genus. The in silico DNA–DNA hybridization value of strain FJAT-25547 with the most closely related species was 22.7 %, <70 %, again indicating they belong to different taxa. The DNA G+C content of strain FJAT-25547 was 39.1 mol%. This taxono-genomics study revealed that strain FJAT-25547 represents a novel species of the genus Bacillus for which the name Bacillus praedii sp. nov. (type strain FJAT-25547=CCTCC AB 2015208=DSM 101002) is proposed.

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of strain FJAT-25547 are KY582959 and LMBX00000000, respectively.

  • Three supplementary tables and four supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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