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Volume 67, Issue 8

sp. nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig ( f. ) Free

Abstract

A Gram-stain-positive, facultatively anaerobic, and catalase- and oxidase-negative bacterial strain designated MOZM2, having 98.4 % 16S rRNA gene sequence identity with DSM 20016, was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the , and genes confirmed as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA–DNA hybridisation revealed a 42 % degree of genetic similarity between the novel strain and DSM 20016. Strain MOZM2 degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2 and DSM 20016 in their proportions of C, C, C, Cω6 and C fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G+C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name sp. nov. is proposed, of which the type strain is MOZM2 (=CCM 8609=DSM 100239=LMG 28780).

  • Received:
  • Accepted:
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Keyword(s): guinea pig , Lactobacillus sp. nov. , MLSA and oral cavity
© 2017 IUMS
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2017-08-01
2024-04-24
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Lactobacillus caviae sp. nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)
Int J Syst Evol Microbiol 67, 2903 (2017); https://doi.org/10.1099/ijsem.0.002044
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