@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002059, author = "Kim, Soo-Jin and Cho, Hayoung and Joa, Jae-Ho and Hamada, Moriyuki and Ahn, Jae-Hyung and Weon, Hang-Yeon and Kwon, Soon-Wo", title = "Nakamurella intestinalis sp. nov., isolated from the faeces of Pseudorhynchus japonicus", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "8", pages = "2970-2974", doi = "https://doi.org/10.1099/ijsem.0.002059", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002059", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polar lipid", keywords = "quinone", keywords = "16S rRNA gene", keywords = "Nakamurella intestinalis", abstract = "One strain, designated 63MJ-1T, was isolated from fresh faeces of broad-winged katydids collected in Jinan-gun, Jeollabuk-do, the Republic of Korea. The organism stained Gram-positive and was an aerobic, non-flagellated and short-rod-shaped bacterium. The organism grew in the range of 4–35 °C (optimum, 28–30 °C) and pH 6.0–9.0 (optimum, pH 7.0), and in the presence of 5 % NaCl (w/v), but not in media containing 7 % NaCl. According to the 16S rRNA gene sequence analysis, strain 63MJ-1T showed the highest sequence similarities with Nakamurella panacisegetis P4-7T (95.9 %), Nakamurella endophytica 2Q3S-4-2T (95.8 %) and Nakamurella multipartita DSM 44233T (95.7 %). Phylogenetic trees also indicated that strain 63MJ-1T formed one robust cluster with members of the genus Nakamurella . The predominant quinone of strain 63MJ-1T was MK-8(H4). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic amino acid. The DNA G+C content was 64.6 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 63MJ-1T represents a novel species of the genus Nakamurella , for which the name Nakamurella intestinalis sp. nov. is proposed. The type strain is 63MJ-1T (=KACC 18662T=NBRC 111844T).", }