1887

Abstract

A novel actinobacterial strain, designated YIM A1135, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive and partially acid-alcohol-fast. Strain YIM A1135 shared 98.3 % 16S rRNA gene sequence similarity with NBRC 103114 and 97.6 % with YIM 30243. DNA–DNA hybridization values between strain YIM A1135 and related type strains of the genus were less than 70 %. In addition, -diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan. The whole-cell sugars were fructose, mannose, galactose and glucose. The major isoprenoid quinone was MK-8(H,), while the major fatty acids (>10 %) were C, Cω9, C 10-methyl and summed feature 3 (Cω7 and/or Cω6). The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were present. The genomic DNA G+C content of strain YIM A1135 was 66.7 mol%. Based on the results of the molecular studies supported by its morphological, physiological, chemotaxnomic and other differential phenotypic characteristics, strain YIM A1135 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is YIM A1135 (=KCTC 39595=CCTCC AA 2017030).

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2017-08-01
2024-03-29
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