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Volume 67, Issue 9

sp. nov., isolated from tree sap Free

Abstract

Two Gram-stain-negative bacterial strains, SAP-32 and SAP-36, were isolated from sap drawn from the from Mount Halla in Jeju, Republic of Korea. The organisms were strictly aerobic, non-sporulating, motile rods and showed growth at 10–30 °C, pH 7–8 and with 0–2 % NaCl. The major isoprenoid quinone was Q-8. The predominant fatty acids were C, cyclo-C, summed feature 3 and C. The polar lipids contained phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminophosphoglycolipid, an unknown glycolipid, an unknown phospholipid and two unknown lipids. The DNA G+C content was 64.4 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that SAP-32 and SAP-36 formed a distinct cluster with members of the genus within the family . Both strains showed 16S rRNA gene sequence similarity of 100 % to each other. The closest relatives of the isolates were (97.08 % sequence similarity), (97.01 %) and (96.73 %). On the basis of data from phenotypic, chemotaxonomic and phylogenetic analyses, SAP-32 (=KCTC 52619=DSM 104039) and SAP-36 (=KCTC 52620=DSM 104072) represent members of a novel species of the genus , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , new taxa, proteobacteria , Pigmentiphaga aceris sp. nov. and polyphasic investigation
© 2017 IUMS
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2017-09-01
2024-04-20
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Pigmentiphaga aceris sp. nov., isolated from tree sap
Int J Syst Evol Microbiol 67, 3198 (2017); https://doi.org/10.1099/ijsem.0.002073
/content/journal/ijsem/10.1099/ijsem.0.002073
/content/journal/ijsem/10.1099/ijsem.0.002073
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