RT Journal Article SR Electronic(1) A1 Dahal, Ram Hari A1 Shim, Dong Seop A1 Kim, JaisooYR 2017 T1 Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 8 SP 3043 OP 3049 DO https://doi.org/10.1099/ijsem.0.002078 PB Microbiology Society, SN 1466-5034, AB During isolation of soil bacteria having antibacterial functions, an aerobic, Gram-stain-positive, oxidase-negative, catalase-positive bacterium, designated strain R434T, was isolated. Strain R434T showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis and significant enzyme-inhibitory capability. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were galactose, arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R434T formed a lineage within the family Pseudonocardiaceae . Strain R434T showed highest sequence similarity with type strains of the genus Actinokineospora , including Actinokineospora guangxiensis Gk-6T (99.4 % sequence similarity), Actinokineospora soli YIM 75948T (98.5 %), Actinokineospora fastidiosa IMSNU 20054T (98.0 %), Actinokineospora cibodasensis ID03-0784T (97.9 %), Actinokineospora terrae IFO 15668T (97.6 %) and Actinokineospora auranticolor IFO 16518T (97.4 %). The predominant respiratory quinone of strain R434T was MK-9(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and unidentified glycolipid. The major cellular fatty acids were iso-C16 : 0, iso-C16 : 1 H and C17 : 1ω6c. The DNA G+C content of strain R434T was 71.6 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R434T represents a novel species of the genus Actinokineospora , for which the name Actinokineospora acnipugnans sp. nov. is proposed. The type strain of ' Actinokineospora acnipugnans' is R434T (=KEMB 9005-403T=KACC 18904T=JCM 31557T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002078