1887

Abstract

A Gram-stain-negative, motile, non-spore-forming, ovoid or rod-shaped bacterium, designated strain B15, was isolated from the culture broth of a marine microalga, sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B15 forms a stable cluster with KCTC 22669 (95.4 % 16S rRNA gene sequence similarity), CB-27A (94.9 %) and HSF11 (94.6 %) in the family Optimal growth of strain B15 was observed at 33 °C, pH 8–9 and in the presence of 3 % (w/v) NaCl. The only detected ubiquinone of strain B15 was Q-10, and the G+C content of the genomic DNA was 66.3 mol%. The major fatty acid profile comprised C cyclo 8, Cω7 ω6 and C iso I/anteiso B. The major polar lipids of strain B15 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unidentified aminolipids, and one unidentified lipid. Comprehensive analyses based on polyphasic characterization of strain B15 indicated that it represents a novel species of a new genusin the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is B15 (=KCTC 52552=MCCC 1K03221).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002081
2017-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3209.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002081&mimeType=html&fmt=ahah

References

  1. Garrity GM, Bell JA, Lilburn T. Family X. Rhodobiaceae fam. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria) New York, NY: Springer; 2005 p. 571
    [Google Scholar]
  2. Albuquerque L, Rainey FA, Pena A, Tiago I, Veríssimo A et al. Tepidamorphus gemmatus gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria. Syst Appl Microbiol 2010; 33:60–66 [View Article][PubMed]
    [Google Scholar]
  3. Wang G, Tang M, Wu H, Dai S, Li T et al. Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122. Int J Syst Evol Microbiol 2016; 66:184–188 [View Article][PubMed]
    [Google Scholar]
  4. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
    [Google Scholar]
  5. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  6. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  9. Swofford D.L. PAUP: Phylogenetic analysis using parsimony, version 3.1.1 Champaign, IL: Illinois Natural History Survey; 1993
    [Google Scholar]
  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  13. Munoz R, Yarza P, Ludwig W, Euzéby J, Amann R et al. Release LTPs104 of the All-Species Living Tree. Syst Appl Microbiol 2011; 34:169–170 [View Article][PubMed]
    [Google Scholar]
  14. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  15. Gerhardt P, Murray RGE, Wood WA, Krieg NR. Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994
    [Google Scholar]
  16. Dong XZ, Cai MY. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  17. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007 pp. 330–393
    [Google Scholar]
  18. Collins MD. Isoprenoid quinones. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 345–401
    [Google Scholar]
  19. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
    [Google Scholar]
  20. Kamekura M. Lipids of extreme halophiles. In Vreeland RH, Hochstein LI. (editors) The Biology of Halophilic Bacteria Boca Raton, FL: CRC Press; 1993 pp. 135–161
    [Google Scholar]
  21. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  22. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  23. Kumar PA, Srinivas TN, Manasa P, Madhu S, Shivaji S. Lutibaculum baratangense gen. nov., sp. nov., a proteobacterium isolated from a mud volcano. Int J Syst Evol Microbiol 2012; 62:2025–2031 [View Article][PubMed]
    [Google Scholar]
  24. Su Y, Han S, Wang R, Yu X, Fu G et al. Microbaculum marinum gen. nov., sp. nov., isolated from deep seawater. Int J Syst Evol Microbiol 2017; 67:812–817 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002081
Loading
/content/journal/ijsem/10.1099/ijsem.0.002081
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error