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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., an actinomycete isolated from roots Free

Abstract

A novel actinomycete, designated strain EURKPP3H10, was isolated from surface-sterilized roots of Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10 was studied using a polyphasic approach. Phylogenetic evaluation based on 16S rRNA gene sequence analysis showed that strain EURKPP3H10 belongs to the genus , with the highest sequence similarity to LIPI11-2-Ac046 (99.2 %). Colonies of strain EURKPP3H10 were orange yellow. Spherical sporangia with motile spores were observed. The strain contained -diaminopimelic acid and acofriose, arabinose, galactose, glucose, mannose, xylose and ribose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C, Cω8, Cω9 and C. The polar lipids of the strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unknown lipids. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including DNA–DNA hybridization, strain EURKPP3H10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EURKPP3H10 (=BCC 77605=NBRC 111482).

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  • Accepted:
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Keyword(s): actinomycete , Cryptosporangium , Eucalyptus camaldulensis and polyphasic taxonomy
© 2017 IUMS
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2017-08-01
2024-04-20
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Cryptosporangium eucalypti sp. nov., an actinomycete isolated from Eucalyptus camaldulensis roots
Int J Syst Evol Microbiol 67, 3077 (2017); https://doi.org/10.1099/ijsem.0.002086
/content/journal/ijsem/10.1099/ijsem.0.002086
/content/journal/ijsem/10.1099/ijsem.0.002086
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