@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002089, author = "Hyeon, Jong Woo and Kim, Kyungchul and Son, Ah Ryeong and Choi, Eunmi and Lee, Sung Kuk and Jeon, Che Ok", title = "Novosphingobium humi sp. nov., isolated from soil of a military shooting range", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "8", pages = "3083-3088", doi = "https://doi.org/10.1099/ijsem.0.002089", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002089", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Novosphingobium humi", keywords = "soil", keywords = "new taxa", keywords = "shooting range", abstract = "A Gram-stain-negative, strictly aerobic bacterium, designated R1-4T, was isolated from soil from a military shooting range in the Republic of Korea. Cells were non-motile short rods, oxidase-positive and catalase-negative. Growth of R1-4T was observed at 15–45 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, pH 7.0). R1-4T contained summed feature 8 (comprising C18 : 1ω7c/C18  : 1ω6c), summed feature 3 (comprising C16 : 1ω7c/C16  : 1ω6c), cyclo-C19 : 0ω8c and C16 : 0 as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, an unknown glycolipid and four unknown lipids were detected as polar lipids. The major polyamine was spermidine. The G+C content of the genomic DNA was 64.4 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that R1-4T formed a tight phylogenetic lineage with Novosphingobium sediminicola HU1-AH51T within the genus Novosphingobium . R1-4T was most closely related to N. sediminicola HU1-AH51T with a 98.8 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between R1-4T and the type strain of N. sediminicola was 37.8±4.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that R1-4T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium humi sp. nov. is proposed. The type strain is R1-4T (=KACC 19094T=JCM 31879T).", }