Chromobacterium sphagni sp. nov., an insecticidal bacterium isolated from Sphagnum bogs

Michael B. Blackburn; Robert R. Farrar Jr.; Michael E. Sparks; Daniel Kuhar; Ashaki Mitchell; Dawn E. Gundersen-Rindal

doi:10.1099/ijsem.0.002127

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f Chromobacterium sphagni sp. nov., an insecticidal bacterium isolated from Sphagnum bogs

Authors: Michael B. Blackburn¹ , Robert R. Farrar Jr.¹ , Michael E. Sparks¹ , Daniel Kuhar¹ , Ashaki Mitchell¹ , Dawn E. Gundersen-Rindal¹
- VIEW AFFILIATIONS
- ¹ Invasive Insect Biocontrol and Behavior Laboratory, USDA, ARS, Bldg 007, Rm 301, BARC-West, Beltsville, MD 20705, USA
*Correspondence: Michael B. Blackburn, [email protected]
First Published Online: 22 August 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3417-3422, doi: 10.1099/ijsem.0.002127
Subject: New taxa

Received: 04/11/2016
Accepted: 02/06/2017
Cover date: 01/09/2017

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Sixteen isolates of Gram-reaction-negative, motile, violet-pigmented bacteria were isolated from Sphagnum bogs in West Virginia and Maine, USA. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genome sequencing of two isolates, IIBBL 14B-1T and IIBBL 37-2 (from West Virginia and Maine, respectively), revealed highly similar genomic sequences. The average nucleotide identity (gANI) calculated for these two isolates was found to be in excess of 99 %, but did not exceed 88 % when comparing either isolate with genomic sequences of Chromobacterium violaceum ATCC 12472T, C. haemolyticum DSM 19808T, C. piscinae ND17, C. subtsugae PRAA4-1T, C. vaccinii MWU205T or C. amazonense CBMAI 310T. Collectively, gANI and 16S rRNA gene sequence comparisons suggested that isolates IIBBL 14B-1T and IIBBL 37-2 were most closely related to C. subtsugae , but represented a distinct species. We propose the name Chromobacterium sphagni sp. nov. for this taxon; the type strain is IIBBL 14B-1T (=NRRL B-67130T=JCM 31882T).

The GenBank/EMBL/DDBJ accession number for the genome assembly of strain IIBBL 14B-1T is GCA_001855555.1.
Two supplementary figures and three supplementary tables are available with the online Supplementary Material.

Keyword(s): insecticidal bacteria, Betaproteobacteria, Sphagnum bog, neisseriales

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