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Abstract

A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1–2 mm in diameter, convex and had an entire margin. The major fatty acids included C, C /Cω6 and Cω7. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA–DNA hybridization between strain AK56 and LMG 5214 and ‘ ’ NIO-S6 showed relatedness values of 39.91 and 23.62 %, respectively. The DNA G+C content of strain AK56 was found to be 50.3 mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that and were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 %, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56 with close relative and . Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56 is described in this study as a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is AK56 (=MTCC 12005=JCM 19193=KCTC 52973).

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2017-09-01
2024-03-29
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