@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002135, author = "Ahn, Joong-Hyeon and Kim, Tae Woon and Kim, Tae-Su and Joung, Yochan and Kim, Seung Bum", title = "Flavobacterium fluminis sp. nov. to accommodate an aerobic, halotolerant and gliding flavobacterium isolated from freshwater", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "8", pages = "3117-3121", doi = "https://doi.org/10.1099/ijsem.0.002135", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002135", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Flavobacterium fluminis", keywords = "Keum river", keywords = "halotolerant", keywords = "freshwater", abstract = "A Gram-stain-negative, aerobic, oxidase-positive, catalase-positive and rod-shaped bacterium designated strain 3R17T was isolated from freshwater. Strain 3R17T produced bright-yellow, circular, convex and smooth colonies on R2A agar, tryptic soy agar, potato dextrose agar, nutrient agar and brain–heart infusion agar media. The strain was motile by gliding. The strain grew at 4–30 °C (optimum, 25 °C), at pH 6–9 (optimum, pH 7) and in the presence of up to 3 % NaCl (optimum, 0 %) on R2A agar. The 16S rRNA gene sequence analysis indicated that 3R17T represents a member of the genus Flavobacterium and is most closely related to Flavobacterium resistens BD-b365T, with a sequence similarity of 97.78 %, but the strain formed a distinct phylogenetic lineage of its own. Fatty acid analysis indicated that a summed feature comprising C16 : 1ω7c and/or C16 : 1ω6c, iso-C15 : 0, iso-C15 : 1G, anteiso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH were the major components (>5 %). Strain 3R17T contained phosphatidylethanolamine (PE) and several unidentified aminolipids as main polar lipids, and MK-6 as the predominant isoprenoid quinone. Flexirubin pigments were not produced. The DNA G+C content was 35.4 mol%. The combination of physiological and chemotaxonomic properties distinguished 3R17T from related species of the genus Flavobacterium . On the basis of polyphasic taxonomy, 3R17T evidently represents a novel species within the genus Flavobacterium , for which the name Flavobacterium fluminis sp. nov. is proposed. The type strain is 3R17T (=KCTC 42062T=JCM 30338T).", }