@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002138, author = "Ma, Li and Xi, Jia-Qin and Cao, Yong-Hong and Wang, Xiao-Yan and Zheng, Shuai-Chao and Yang, Cheng-Gang and Yang, Ling-ling and Mi, Qi-Li and Li, Xue-Mei and Zhu, Ming-Liang and Mo, Ming-He", title = "Bacillus endozanthoxylicus sp. nov., an endophytic bacterium isolated from Zanthoxylum bungeanum Maxim leaves", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3699-3705", doi = "https://doi.org/10.1099/ijsem.0.002138", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002138", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Endophytes", keywords = "Zanthoxylum bungeanum Maxim", keywords = "Bacillus endozanthoxylicus sp. nov.", keywords = "polyphasic taxonomy", abstract = "A Gram-stain-positive, rod-shaped, motile bacterium, designated as 1404T, was isolated from leaves of Chinese red pepper (Huajiao) (Zanthoxylum bungeanum Maxim) collected from Gansu, north-west China. Spores were not observed under a range of conditions. Strain 1404T was observed to grow at 15–45 °C and pH 6.0–10.0 and in presence of 0–5 % (w/v) NaCl concentration. The cell wall of strain 1404T was found to contain meso-diaminopimelic acid, and the predominant respiratory quinone was identified as MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as well as three unidentified polar lipids. The major fatty acids profile of strain 1404T consisted of iso-C15 : 0 (25.6 %), anteiso-C15 : 0 (18.4 %) and iso-C14 : 0 (12.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1404T was affiliated to the genus Bacillus and was closely related to Bacillus oryzisoli 1DS3-10T, Bacillus benzoevorans DSM 5391T and Bacillus circulans DSM 11T with sequence similarity of 98.3, 98.2 and 96.9 %, respectively. The G+C content of the genomic DNA was determined to be 39.4 mol%. DNA–DNA hybridization values indicated that relatedness between strain 1404T and the type strains of closely related species of the genus Bacillus was below 41 %. Therefore, on the basis of the data from the polyphasic taxonomic study presented, strain 1404T represents a novel species of the genus Bacillus , for which the name proposed is Bacillus endozanthoxylicus sp. nov. The type strain is 1404T (=CCTCC AB 2017021T=KCTC 33827T).", }