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f Vibrio palustris sp. nov. and Vibrio spartinae sp. nov., two novel members of the Gazogenes clade, isolated from salt-marsh plants (Arthrocnemum macrostachyum and Spartina maritima)

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Vibrio palustris sp. nov. and Vibrio spartinae sp. nov., two novel members of the Gazogenes clade, isolated from salt-marsh plants (Arthrocnemum macrostachyum and Spartina maritima), Page 1 of 1

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  • Two bacterial strains, EAod9 and SMJ21, isolated from salt-marsh plants, were determined to be related to species of the genus Vibrio from from 16S rRNA sequence comparisons. Their closest phylogenetic relatives are members of the Gazogenes clade, Vibrio mangrovi and Vibrio rhizosphaerae , which show the greatest similarity to the SMJ21rRNA sequence (97.3 and 97.1 %, respectively), while EAod9 had less than 97.0 % similarity to any other species of the genus Vibrio . Both strains share the basic characteristics of the genus Vibrio , as they are Gram-stain negative, motile, slightly halophilic, facultatively anaerobic bacteria. In addition, they are oxidase-negative and unable to grow on TCBS Agar; they grow between 15 to 26 °C, pH 6 to 8 and in up to 10 % (w/v) total salinity. They produce indol, are positive in the Voges-Proskauer test and are negative for arginine dihydrolase, lysine and ornithine decarboxylases. Strain SMJ21 is aerogenic and red-pigmented, due to prodigiosin production, while strain EAod9 ferments glucose without gas and is not pigmented. The major cellular fatty acids of both novel strains were C16 : 1ω7c/C16  : 1ω6c and C16 : 0. WGSobtained for both strains, along with the other five members of the clade, allowed the determination of ANI indexes and in silico estimations of DDH values, which confirmed that the two strains represent two novel species of the genus Vibrio: Vibrio palustris sp. nov. (with EAod9=CECT 9027=LMG 29724 as the proposed type strain) and Vibrio spartinae sp. nov. (with SMJ21=CECT 9026=LMG 29723 as the proposed type strain).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of EAod9 and SMJ21 are KU320862 and KX583607, respectively. Whole genome sequences determined in this study are publicly available under the accesion numbers indicated in parentheses: Vibrio spartinae CECT 9026 (FSSB0100000); V. palustris CECT 9027 (FUFT0100000); Vibrio gazogenes CECT 5068 (FULF0100000); Vibrio aerogenes CECT 7868 (FQXZ0100000); Vibrio ruber CECT 7878 (FULE0100000), Vibrio quintilis CECT 7734 (FRFG0100000) and Vibrio mangrovi CECT 7927 (FXXI0100000).

  • Three supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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2017-08-31
2019-02-20
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