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f Promicromonospora callitridis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized root of an Australian native pine tree

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  • Authors: Onuma Kaewkla1​,2​ , Christopher M. M. Franco2​
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    • 1​ 1​Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham Province 44150, Thailand 2​ 2​Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park SA 5042, Australia
  • *Correspondence: Christopher M. M. Franco, [email protected]
  • First Published Online: 04 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3559-3563, doi: 10.1099/ijsem.0.002165
  • Subject: New taxa
  • Received:
  • Accepted:
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Promicromonospora callitridis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized root of an Australian native pine tree, Page 1 of 1

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  • A new strain of the genus Promicromonospora , CAP94, was isolated from the surface sterilized root of Callitrispreissii (Australian native pine tree). This strain was a Gram-stain-positive, aerobic actinobacterium with hyphae breaking up into fragments which were non-motile, rod-like, coccoid elements. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of the family Promicromonospora ceae , and most closely to Promicromonospora sukumoe NBRC 14650 (99.4 %), Promicromonospora kroppenstedtii DSM 19349 (99.2 %) and Promicromonosporaaerolata V54A (99.1 %). Chemotaxonomic data including cell-wall components, major menaquinone and major fatty acids confirmed the affiliation of strain CAP94 to the genus Promicromonospora . The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA–DNA hybridization, allowed the genotypic and phenotypic differentiation of strain CAP94 and the closest species with validly published names. The name proposed for the new species is Promicromonospora callitridis sp. nov. The type strain is CAP94 (=DSM 103339=TBRC 6025).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAP94 is GU434237.

  • Three supplementary figures and two supplementary tables are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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