1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile bacterium, designated D4N98, was isolated from a desert soil near Glendambo, Australia. The taxonomic position of strain D4N98 was investigated using a polyphasic approach. Strain D4N98 shared 97 % 16S rRNA gene sequence similarity with the only reference strain of the genus ( N6PO6), and less than 96 % similarity with other species of genera in the family Enterobacteriaceae with validly published names. Phylogenetic analysis, based on 16S rRNA, , , genes and a concatenated sequence comprising 37 single copy marker genes, as well as chemotaxonomic data (major polar lipids: phosphatidyl ethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid, and a glycolipid. Major fatty acids (>10 %): C, C cyclo, summed feature 2 (C 3-OH and/or iso-C I), summed feature 3 (Cω7 and/or Cω6), summed feature 8 (C ω7 and/or Cω6) support the affiliation of this strain to the genus . The results of DNA–DNA hybridisation plus physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain D4N98 from the other species of the genus with validly published names. Therefore, strain D4N98 represents a novel species, for which the name sp. nov. is proposed. The type strain is D4N98 (LMG 30066=DSM 105030).

Keyword(s): Izhakiella and taxonogenomic
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2017-11-01
2024-03-28
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