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- Volume 67, Issue 10
- Article

f Borrelia lanei sp. nov. extends the diversity of Borrelia species in California
- Authors: Gabriele Margos1 , Natalia Fedorova2 , Joyce E. Kleinjan2 , Christine Hartberger1 , Tom G. Schwan3 , Andreas Sing1 , Volker Fingerle1
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1 1German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany 2 2Department of Environmental Science, University of California, Policy and Management, Berkeley, CA, USA 3 3Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Disease and National Institutes of Health, Rocky Mountain Laboratories (RML), Hamilton, MT, USA
- *Correspondence: Gabriele Margos [email protected]
- First Published Online: 08 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3872-3876, doi: 10.1099/ijsem.0.002214
- Subject: New taxa - Other Bacteria
- Received:
- Accepted:
- Cover date:




Borrelia lanei sp. nov. extends the diversity of Borrelia species in California, Page 1 of 1
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The diversity of Borrelia species discovered in California appears to be particularly high. A divergent group of Borrelia strains collected from Ixodes ticks in California was described by Postic and co-workers and designated ‘genomospecies 2’ (Postic D, Garnier M, Baranton G. Int J Med Microbiol 2007;297:263–271; Postic D, Ras NM, Lane RS, Hendson M, Baranton G. J Clin Microbiol 1998;36:3497–3504). We performed multilocus sequence analysis (MLSA) using eight housekeeping loci (clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA) on 12 strains of this Borrelia genospecies to confirm that these strains form a distinct group within the Borrelia burgdorferi s. l. complex (Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V et al. Ticks Tick Borne Dis 2010;1:151–158). Phylogenetic and genetic distance analyses based on sequences of the MLSA housekeeping genes corroborated the distinctness of this group; genetic distances to all other members of the B. burgdorferi s.l. complex were 96 % or lower. We propose the name Borrelia lanei sp. nov. for this genospecies in honor of Professor Robert S. Lane, University of California Berkeley, for his contributions to Borrelia and tick research. The type strain for Borrelia lanei sp. nov., strain CA28-91T, has been deposited to two culture collections (=DSM 17992T=CIP 109135T).
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The GenBank/EMBL/DDBJ accession numbers for type strain CA28-91 for sequences are available via GenBank accession nos. rrf-rrl AJ006375; flaB DQ393351; hbb DQ393371; groEL DQ393392; recA DQ393410; sequences for the MLST housekeeping genes are available for MLST ST 719-730 at the Borrelia MLST website at www.pubMLST.org (ID numbers 1949−1960).
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One supplementary table is available with the online Supplementary Material.
- Keyword(s): MLSA, species complex, Borrelia burgdorferi sensu lato, tick-borne bacteria
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