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f Borrelia lanei sp. nov. extends the diversity of Borrelia species in California

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  • Authors: Gabriele Margos1​ ,  Natalia Fedorova2​ ,  Joyce E. Kleinjan2​ ,  Christine Hartberger1​ ,  Tom G. Schwan3​ ,  Andreas Sing1​ , Volker Fingerle1​
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    • 1​ 1​German National Reference Centre for Borrelia at the Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, Germany 2​ 2​Department of Environmental Science, University of California, Policy and Management, Berkeley, CA, USA 3​ 3​Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Disease and National Institutes of Health, Rocky Mountain Laboratories (RML), Hamilton, MT, USA
  • *Correspondence: Gabriele Margos [email protected]
  • First Published Online: 08 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3872-3876, doi: 10.1099/ijsem.0.002214
  • Subject: New taxa - Other Bacteria
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  • The diversity of Borrelia species discovered in California appears to be particularly high. A divergent group of Borrelia strains collected from Ixodes ticks in California was described by Postic and co-workers and designated ‘genomospecies 2’ (Postic D, Garnier M, Baranton G. Int J Med Microbiol 2007;297:263–271; Postic D, Ras NM, Lane RS, Hendson M, Baranton G. J Clin Microbiol 1998;36:3497–3504). We performed multilocus sequence analysis (MLSA) using eight housekeeping loci (clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA) on 12 strains of this Borrelia genospecies to confirm that these strains form a distinct group within the Borrelia burgdorferi s. l. complex (Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V et al. Ticks Tick Borne Dis 2010;1:151–158). Phylogenetic and genetic distance analyses based on sequences of the MLSA housekeeping genes corroborated the distinctness of this group; genetic distances to all other members of the B. burgdorferi s.l. complex were 96 % or lower. We propose the name Borrelia lanei sp. nov. for this genospecies in honor of Professor Robert S. Lane, University of California Berkeley, for his contributions to Borrelia and tick research. The type strain for Borrelia lanei sp. nov., strain CA28-91, has been deposited to two culture collections (=DSM 17992=CIP 109135).

  • The GenBank/EMBL/DDBJ accession numbers for type strain CA28-91 for sequences are available via GenBank accession nos. rrf-rrl AJ006375; flaB DQ393351; hbb DQ393371; groEL DQ393392; recA DQ393410; sequences for the MLST housekeeping genes are available for MLST ST 719-730 at the Borrelia MLST website at www.pubMLST.org (ID numbers 1949−1960).

  • One supplementary table is available with the online Supplementary Material.

  • 1. Kurtenbach K, Hanincová K, Tsao JI, Margos G, Fish D et al. Fundamental processes in the evolutionary ecology of Lyme borreliosis. Nat Rev Microbiol 2006;4:660–669 [CrossRef][PubMed]
     [Google Scholar]
  • 2. Brown RN, Lane RS. Lyme disease in California: a novel enzootic transmission cycle of Borrelia burgdorferi. Science 1992;256:1439–1442 [CrossRef][PubMed]
     [Google Scholar]
  • 3. Brown RN, Lane RS. Reservoir competence of four chaparral-dwelling rodents for Borrelia burgdorferi in California. Am J Trop Med Hyg 1996;54:84–91 [CrossRef][PubMed]
     [Google Scholar]
  • 4. Schwan TG, Schrumpf ME, Karstens RH, Clover JR, Wong J et al. Distribution and molecular analysis of lyme disease spirochetes, Borrelia burgdorferi, isolated from ticks throughout California. J Clin Microbiol 1993;31:3096–3108[PubMed]
     [Google Scholar]
  • 5. Postic D, Garnier M, Baranton G. Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates-description of Borrelia californiensis sp. nov., and genomospecies 1 and 2. Int J Med Microbiol 2007;297:263–271 [CrossRef][PubMed]
     [Google Scholar]
  • 6. Postic D, Ras NM, Lane RS, Hendson M, Baranton G. Expanded diversity among Californian Borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127). J Clin Microbiol 1998;36:3497–3504[PubMed]
     [Google Scholar]
  • 7. Margos G, Vollmer SA, Cornet M, Garnier M, Fingerle V et al. A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl Environ Microbiol 2009;75:5410–5416 [CrossRef][PubMed]
     [Google Scholar]
  • 8. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
     [Google Scholar]
  • 9. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
     [Google Scholar]
  • 10. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
     [Google Scholar]
  • 11. Kingry LC, Batra D, Replogle A, Rowe LA, Pritt BS et al. Whole genome sequence and comparative genomics of the novel Lyme Borreliosis causing pathogen, Borrelia mayonii. PLoS One 2016;11:e0168994 [CrossRef][PubMed]
     [Google Scholar]
  • 12. Pritt BS, Respicio-Kingry LB, Sloan LM, Schriefer ME, Replogle AJ et al. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States. Int J Syst Evol Microbiol 2016;66:4878–4880 [CrossRef][PubMed]
     [Google Scholar]
  • 13. Fedorova N, Kleinjan JE, James D, Hui LT, Peeters H et al. Remarkable diversity of tick or mammalian-associated Borreliae in the metropolitan San Francisco Bay area, California. Ticks Tick Borne Dis 2014;5:951–961 [CrossRef][PubMed]
     [Google Scholar]
  • 14. Girard YA, Travinsky B, Schotthoefer A, Fedorova N, Eisen RJ et al. Population structure of the Lyme Borreliosis spirochete Borrelia burgdorferi in the western black-legged tick (Ixodes pacificus) in Northern California. Appl Environ Microbiol 2009;75:7243–7252 [CrossRef][PubMed]
     [Google Scholar]
  • 15. Scott JD, Clark KL, Foley JE, Anderson JF, Durden LA et al. Detection of Borrelia genomospecies 2 in Ixodes spinipalpis ticks collected from a rabbit in Canada. J Parasitol 2017;103:38–46 [CrossRef][PubMed]
     [Google Scholar]
  • 16. Johnson RC, Schmid GP, Hyde FW, Steigerwalt AG, Brenner DJ. Borrelia burgdorferi sp. nov.: etiologic agent of Lyme disease. Int J Syst Bacteriol 1984;34:496–497 [CrossRef]
     [Google Scholar]
  • 17. Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V et al. Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. Ticks Tick Borne Dis 2010;1:151–158 [CrossRef][PubMed]
     [Google Scholar]
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