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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

Description of subsp. subsp. nov., isolated from cattle (), emended description of and creation of subsp. subsp. nov. Free

Abstract

Three rapidly growing mycobacterial strains, QIA-37, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (). These strains were previously shown to be phylogenetically distinct but closely related to ATCC 35752 by taxonomic approaches targeting three genes (16S rRNA, and ) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7 % sequence similarity with that of the type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including and , revealed a phylogenetic cluster of these strains with . DNA–DNA hybridization values of 78.2 % between QIA-37 and indicated that it belongs to but is a novel subspecies distinct from . Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06 % average nucleotide identity (ANI) value with , slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of . Therefore, we propose an emended description of , and descriptions of subsp. subsp. nov. and subsp. subsp. nov. are presented; strains ATCC 35752(=CCUG 47445=CIP 104535=DSM 43804=JCM 6388=NCTC 946) and QIA-37 (=KCTC 39630=JCM 30986) are the type strains of the two novel subspecies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): multilocus sequence typing , Mycobacterium chelonae , Mycobacterium chelonae subsp. bovis , Mycobacterium chelonae subsp. chelonae and rapid growing mycobacteria
© 2017 IUMS
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2017-10-01
2024-04-19
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Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov.
Int J Syst Evol Microbiol 67, 3882 (2017); https://doi.org/10.1099/ijsem.0.002217
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