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f Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov.

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  • Authors: Byoung-Jun Kim1​ ,  Ga-Na Kim1​ ,  Bo-Ram Kim1​ ,  Che Ok Jeon2​ ,  Joseph Jeong3​ ,  Seon Ho Lee3​ ,  Ji-Hun Lim3​ ,  Seung-Heon Lee4​ ,  Chang Ki Kim4​ ,  Yoon-Hoh Kook1​ , Bum-Joon Kim1​
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    • 1​ 1​Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea 2​ 2​School of Biological Sciences, Chung-Ang University, Seoul, Republic of Korea 3​ 3​Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea 4​ 4​Korean Institute of Tubercuosis, Chungbuk, Republic of Korea
  • *Correspondence: Bum-Joon Kim [email protected]
  • First Published Online: 12 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3882-3887, doi: 10.1099/ijsem.0.002217
  • Subject: New taxa - Actinobacteria
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Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov., Page 1 of 1

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  • Three rapidly growing mycobacterial strains, QIA-37, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752 by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7 % sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae . DNA–DNA hybridization values of 78.2 % between QIA-37 and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae . Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06 % average nucleotide identity (ANI) value with M. chelonae , slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae . Therefore, we propose an emended description of Mycobacterium chelonae , and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752(=CCUG 47445=CIP 104535=DSM 43804=JCM 6388=NCTC 946) and QIA-37 (=KCTC 39630=JCM 30986) are the type strains of the two novel subspecies.

© 2017 IUMS | Published by the Microbiology Society

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