1887

Abstract

A Gram-stain-positive, aerobic, non-spore-forming, atrichous and short rod-shaped endophytic actinomycete, designated strain BGMRC 2075, was isolated from the leaves of , and was subjected to polyphasic characterization to unravel its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BGMRC 2075 belongs to the genus ,showing the highest 16S rRNA gene sequence similarity to JC2056 (96.1 %), MSL-28 (95.1 %) and MSL-26 (95.1 %). The predominant cellular fatty acids of strain BGMRC 2075 were iso-C, Cω8 and C. The major menaquinone was MK-8(H). The diagnostic diamino acid in the cell-wall peptidoglycan was -2,6-diaminopimelic acid. The predominant cell-wall sugars were composed of ribose and glucose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, five unknown phospholipids, one phospholipid of unknown structure containing glucosamine, and an unknown polar lipid. The DNA G+C content was 70.8 mol%. All these data support the allocation of the novel strain to the genus . The results of physiological and biochemical characterization allow the phenotypic differentiation of strain BGMRC 2075 from JC2056, MSL-28 and MSL-26. Strain BGMRC 2075 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is BGMRC 2075 (=KCTC 39886=DSM 104480).

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2017-10-01
2024-03-29
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