@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002218, author = "Liu, Jun and Li, Fei and Gao, Cheng-Hai and Han, Yu and Hao, Huili and Yi, Xiang-Xi and Huang, Ri-Ming", title = "Nocardioides kandeliae sp. nov., an endophytic actinomycete isolated from leaves of Kandelia candel", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3888-3893", doi = "https://doi.org/10.1099/ijsem.0.002218", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002218", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "endophytic actinomycete", keywords = "Nocardioides kandeliae", keywords = "Kandelia candel", abstract = "A Gram-stain-positive, aerobic, non-spore-forming, atrichous and short rod-shaped endophytic actinomycete, designated strain BGMRC 2075T, was isolated from the leaves of Kandelia candel, and was subjected to polyphasic characterization to unravel its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BGMRC 2075T belongs to the genus Nocardioides ,showing the highest 16S rRNA gene sequence similarity to Nocardioides aestuarii JC2056T (96.1 %), Nocardioides agariphilus MSL-28T (95.1 %) and Nocardioides islandiensis MSL-26T (95.1 %). The predominant cellular fatty acids of strain BGMRC 2075T were iso-C16 : 0, C17 : 1ω8c and C17 : 0. The major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were composed of ribose and glucose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, five unknown phospholipids, one phospholipid of unknown structure containing glucosamine, and an unknown polar lipid. The DNA G+C content was 70.8 mol%. All these data support the allocation of the novel strain to the genus Nocardioides . The results of physiological and biochemical characterization allow the phenotypic differentiation of strain BGMRC 2075T from N. aestuarii JC2056T, N. agariphilus MSL-28T and N. islandiensis MSL-26T. Strain BGMRC 2075T represents a novel species of the genus Nocardioides , for which we propose the name Nocardioides kandeliae sp. nov. The type strain is BGMRC 2075T (=KCTC 39886T=DSM 104480T).", }