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- Volume 67, Issue 10
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f Lactobacillus curtus sp. nov., isolated from beer in Finland
- Authors: Yuki Asakawa1 , Nobuchika Takesue1 , Shizuka Asano1 , Satoshi Shimotsu1 , Kazumaru Iijima1 , Koji Suzuki2 , Yasuo Motoyama1 , Masayuki Aizawa1
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- VIEW AFFILIATIONS
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1 1Research Laboratories for Alcohol Beverages, Asahi Breweries, Ltd, 1-21, Midori 1-chome, Moriya-shi, Ibaraki, 302-0106, Japan 2 2Quality Control Center, Asahi Breweries, Ltd, 1-21, Midori 1-chome, Moriya-shi, Ibaraki, 302-0106, Japan
- *Correspondence: Yuki Asakawa [email protected], Nobuchika Takesue [email protected]
- First Published Online: 12 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3899-3906, doi: 10.1099/ijsem.0.002220
- Subject: New taxa - Firmicutes and Related Organisms
- Received:
- Accepted:
- Cover date:




Lactobacillus curtus sp. nov., isolated from beer in Finland, Page 1 of 1
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A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae . However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S–23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis . Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA–DNA relatedness between VTT E-94560 and these two type strains was 4 % ( L. rossiae JCM 16176T) and 12 % (L. siliginins JCM 16155T), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560T (=JCM 31185T).
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One supplementary table and one supplementary figure are available with the online Supplementary Material.
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The DDBJ accession numbers for the sequences reported in this paper are LC093898 (16S rRNA gene sequence of VTT E-94560T), LC229691 (16S rRNA gene sequence of strain ABBC636), LC229692 (16S rRNA gene sequence of strain ABBC637), LC229693 (16S rRNA gene sequence of strain ABBC638), LC229694 (16S rRNA gene sequence of strain ABBC639), LC171388 (pheS partial gene sequence of VTT E-94560T), LC171389 (rpoA partial gene sequence of VTT E-94560T), LC171390 (rpoA partial gene sequence of L. siliginis JCM16155T), LC171391 (ITS region of VTT E-94560T), LC198583 (ITS region of L. rossiae JCM16176T) and LC171392 (ITS region of L. siliginis JCM16155T).
- Keyword(s): Lactobacillus cultus, beer
© 2017 IUMS | Published by the Microbiology Society
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1. Suzuki K. 125th Anniversary Review: Microbiological instability of beer caused by spoilage bacteria. J Inst Brew 2011; 117: 131– 155 [CrossRef]
-
2. Corsetti A, Settanni L, van Sinderen D, Felis GE, Dellaglio F et al. Lactobacillus rossii sp. nov., isolated from wheat sourdough. Int J Syst Evol Microbiol 2005; 55: 35– 40 [CrossRef] [PubMed]
-
3. Hutzler M, Müller-Auffermann K, Koob J, Riedl R, Jacob F. Beer spoiling microorganisms – a current overview. Brauwelt Int 2013; 31: 23– 25
-
4. Koob J, Jacob F, Grammer M, Kleucker A, Riedl R et al. PCR-analysen bierschädlicher bakterien 2012 und 2013. Brauwelt 2014; 10: 288– 290
-
5. Aslam Z, Im WT, Ten LN, Lee MJ, Kim KH et al. Lactobacillus siliginis sp. nov., isolated from wheat sourdough in South Korea. Int J Syst Evol Microbiol 2006; 56: 2209– 2213 [CrossRef] [PubMed]
-
6. Sami M, Yamashita H, Kadokura H, Kitamoto K, Yoda K et al. A new and rapid method for determination of beer-spoilage ability of lactobacilli. J Am Soc Brew Chem 1997; 55: 137– 140
-
7. Suzuki K, Iijima K, Ozaki K, Yamashita H. Isolation of a hop-sensitive variant of Lactobacillus lindneri and identification of genetic markers for beer spoilage ability of lactic acid bacteria. Appl Environ Microbiol 2005; 71: 5089– 5097 [CrossRef] [PubMed]
-
8. Suzuki K, Iijima K, Sakamoto K, Sami M, Yamashita H. A review of hop resistance in beer spoilage lactic acid bacteria. J Inst Brew 2006; 112: 173– 191 [CrossRef]
-
9. Bruce JL, Hubner RJ, Cole EM, Mcdowell CI, Webster JA. Sets of EcoRI fragments containing ribosomal RNA sequences are conserved among different strains of Listeria monocytogenes. Proc Natl Acad Sci USA 1995; 92: 5229– 5233 [CrossRef] [PubMed]
-
10. Hubner RJ, Cole EM, Bruce JL, Mcdowell CI, Webster JA. Types of Listeria monocytogenes predicted by the positions of EcoRI cleavage sites relative to ribosomal RNA sequences. Proc Natl Acad Sci USA 1995; 92: 5234– 5238 [CrossRef] [PubMed]
-
11. Motoyama Y, Funahashi W, Ogata T. Characterization of Lactobacillus spp. by ribotyping. J Am Soc Brew Chem 2000; 58: 1– 3
-
12. Olsen I, Johnson JL, Moore LV, Moore WE. Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the human gingival crevice and emended descriptions of Lactobacillus minutus and Streptococcus parvulus. Int J Syst Bacteriol 1991; 41: 261– 266 [CrossRef] [PubMed]
-
13. von Wintzingerode F, Selent B, Hegemann W, Göbel UB. Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium. Appl Environ Microbiol 1999; 65: 283– 286 [PubMed]
-
14. Baker GC, Smith JJ, Cowan DA. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 2003; 55: 541– 555 [CrossRef] [PubMed]
-
15. Jiang H, Dong H, Zhang G, Yu B, Chapman LR et al. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Appl Environ Microbiol 2006; 72: 3832– 3845 [CrossRef] [PubMed]
-
16. Dethlefsen L, Huse S, Sogin ML, Relman DA. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 2008; 6: e280 [CrossRef] [PubMed]
-
17. Dobson CM, Deneer H, Lee S, Hemmingsen S, Glaze S et al. Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 2002; 52: 2003– 2010 [CrossRef] [PubMed]
-
18. Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P et al. Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 2005; 151: 2141– 2150 [CrossRef] [PubMed]
-
19. Naser SM, Dawyndt P, Hoste B, Gevers D, Vandemeulebroecke K et al. Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 2007; 57: 2777– 2789 [CrossRef] [PubMed]
-
20. Scheirlinck I, van der Meulen R, Vrancken G, de Vuyst L, Settanni L et al. Polyphasic taxonomic characterization of Lactobacillus rossiae isolates from Belgian and Italian sourdoughs reveals intraspecific heterogeneity. Syst Appl Microbiol 2009; 32: 151– 156 [CrossRef] [PubMed]
-
21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
-
22. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673– 4680 [CrossRef] [PubMed]
-
23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
-
24. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64: 346– 351 [CrossRef] [PubMed]
-
25. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33: 152– 155
-
26. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12: 635– 645 [CrossRef] [PubMed]
-
27. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3: 208– 218 [CrossRef]
-
28. Hamamoto M, Nakase T. Ballistosporous yeasts found on the surface of plant materials collected in New Zealand. 1. Six new species in the genus Sporobolomyces. Antonie van Leeuwenhoek 1995; 67: 151– 171 [PubMed] [Crossref]
-
29. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39: 224– 229 [CrossRef]
-
30. Rosselló-Mora R, Amann R. The species concept for prokaryotes. FEMS Microbiol Rev 2001; 25: 39– 67 [CrossRef] [PubMed]
-
31. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44: 846– 849 [CrossRef]
-
32. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [Crossref]

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