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f Lactobacillus curtus sp. nov., isolated from beer in Finland

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Lactobacillus curtus sp. nov., isolated from beer in Finland, Page 1 of 1

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  • A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae . However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176 with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S–23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis . Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA–DNA relatedness between VTT E-94560 and these two type strains was 4 % ( L. rossiae JCM 16176) and 12 % (L. siliginins JCM 16155), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560 (=JCM 31185).

  • One supplementary table and one supplementary figure are available with the online Supplementary Material.

  • The DDBJ accession numbers for the sequences reported in this paper are LC093898 (16S rRNA gene sequence of VTT E-94560), LC229691 (16S rRNA gene sequence of strain ABBC636), LC229692 (16S rRNA gene sequence of strain ABBC637), LC229693 (16S rRNA gene sequence of strain ABBC638), LC229694 (16S rRNA gene sequence of strain ABBC639), LC171388 (pheS partial gene sequence of VTT E-94560), LC171389 (rpoA partial gene sequence of VTT E-94560), LC171390 (rpoA partial gene sequence of L. siliginis JCM16155), LC171391 (ITS region of VTT E-94560), LC198583 (ITS region of L. rossiae JCM16176) and LC171392 (ITS region of L. siliginis JCM16155).

© 2017 IUMS | Published by the Microbiology Society

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2019-02-24
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