@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002220, author = "Asakawa, Yuki and Takesue, Nobuchika and Asano, Shizuka and Shimotsu, Satoshi and Iijima, Kazumaru and Suzuki, Koji and Motoyama, Yasuo and Aizawa, Masayuki", title = "Lactobacillus curtus sp. nov., isolated from beer in Finland", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3899-3906", doi = "https://doi.org/10.1099/ijsem.0.002220", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002220", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Lactobacillus cultus", keywords = "beer", abstract = "A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae . However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S–23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis . Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA–DNA relatedness between VTT E-94560 and these two type strains was 4 % ( L. rossiae JCM 16176T) and 12 % (L. siliginins JCM 16155T), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560T (=JCM 31185T).", }