@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002227, author = "Pascual, Javier and García-López, Marina and González, Ignacio and Genilloud, Olga", title = "Luteolibacter gellanilyticus sp. nov., a gellan-gum-degrading bacterium of the phylum Verrucomicrobia isolated from miniaturized diffusion chambers", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3951-3959", doi = "https://doi.org/10.1099/ijsem.0.002227", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002227", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "diffusion sandwich system", keywords = "Luteolibacter", keywords = "Verrucomicrobia", abstract = "A novel chemo-organoheterotrophic bacterium, strain CB-286403T, was isolated from a Mediterranean forest soil, collected at Sierra de Tejeda, Almijara and Alhama Natural Park, Spain, by using the Diffusion Sandwich System, a device with 384 miniature diffusion chambers. The 16S rRNA gene sequence analyses identified the isolate as a member of the genus Luteolibacter where the type strains Luteolibacter pohnpeiensis A4T-83T (GenBank acc. no. AB331895), Luteolibacter yonseiensis EBTL01T (JQ319003), Luteolibacter luojiensis DR4-30T (JN630810) and Luteolibacter algae A5J-41-2T (AB331893) were the closest relatives with similarities of 97.0, 96.3, 96.3 and 94.5 %, respectively. The novel isolate was characterized as a Gram-stain-negative, non-motile, short-rod-shaped bacterium. The strain showed a positive response for catalase and cytochrome-c oxidase, divided by binary fission and/or budding, and exhibited an aerobic metabolism. Strain CB-286403T showed a mesophilic and neutrophilic growth range and showed a nutritional preference for simple sugars and complex protein substrates. Major fatty acids included iso-C14 : 0, C16 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and anteiso-C15 : 0. The predominant respiratory quinone was MK-9. Polar lipids comprised major amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and minor amounts of three unidentified lipids, a glycolipid, a phospholipid and a phosphoglycolipid. Based on a polyphasic taxonomic characterization, strain CB-286403T represents a novel species of the genus Luteolibacter , for which the name Luteolibacter gellanilyticus sp. nov. is proposed. The type strain is CB-286403T (=DSM 28998T=CECT 8659T).", }