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- Volume 67, Issue 10
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f Lentibacillus sediminis sp. nov., isolated from a marine saltern
- Authors: Ling-Yun Guo1,2 , Nan-Nan Wang1,2 , Xiao-Qun Wang1,2 , Guan-Jun Chen1,2 , Zong-Jun Du1,2
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1 1State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China 2 2College of Marine Science, Shandong University, Weihai 264209, PR China
- *Correspondence: Zong-Jun Du [email protected]
- First Published Online: 12 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 3946-3950, doi: 10.1099/ijsem.0.002228
- Subject: New taxa - Firmicutes and Related Organisms
- Received:
- Accepted:
- Cover date:




Lentibacillus sediminis sp. nov., isolated from a marine saltern, Page 1 of 1
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A novel, Gram-stain-positive, moderately halophilic, endospore-forming, motile, facultatively anaerobic and rod-shaped strain, designated 0W14T, was isolated from a marine saltern of Wendeng, China. Optimal growth occurred at 37 °C, pH 7.5 and with 6.0 % (w/v) NaCl. MK-7 was the sole respiratory quinone and the peptidoglycan type of 0W14T was A4β l-Orn–d-Glu. The major cellular fatty acid (>10.0 %) in strain 0W14T was anteiso-C15 : 0. The polar lipid profile of strain 0W14T consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and four unknown phospholipids. The genomic DNA G+C content of the strain was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0W14T forms a phylogenetic lineage with members of the genus Lentibacillus within the family Bacillaceae . Based on data from the current polyphasic study, the isolate is proposed to represent a novel species of genus Lentibacillus , for which the name Lentibacillus sediminis sp. nov. is proposed. The type strain is 0W14T (=KCTC 33835T=MCCC 1H00171T).
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and whole-genome shotgun project of strain 0W14T are KX815122 and NIXK00000000, respectively.
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Three supplementary figures are available with the online Supplementary Material.
- Keyword(s): Lentibacillus, moderately halophilic bacteria, polyphasic taxonomy
© 2017 IUMS | Published by the Microbiology Society
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1. Kushner D, Kamekura M. Physiology of halophilic eubacteria. In Rodriguez-Valera F. (editor) Halophilic Bacteria Boca Raton, FL: CRC Press; 1988; pp. 109– 140
-
2. Yoon JH, Kang KH, Park YH. Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 2002; 52: 2043– 2048 [CrossRef] [PubMed]
-
3. Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T et al. Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 2006; 56: 1859– 1863 [CrossRef] [PubMed]
-
4. Oh YJ, Lee HW, Lim SK, Kwon MS, Lee J et al. Lentibacillus kimchii sp. nov., an extremely halophilic bacterium isolated from kimchi, a Korean fermented vegetable. Antonie van Leeuwenhoek 2016; 109: 869– 876 [CrossRef] [PubMed]
-
5. Lane DJ. 16S/23S rRNA sequencing. In Goodfellow M, Stackebrandt E. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp. 115– 147
-
6. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998; 64: 795– 799 [PubMed]
-
7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
-
8. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28: 1823– 1829 [CrossRef] [PubMed]
-
9. Yarza P, Richter M, Peplies J, Euzeby J, Amann R et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 2008; 31: 241– 250 [CrossRef] [PubMed]
-
10. Westram R, Bader K, Pruesse E, Kumar Y, Meier H et al. ARB: a software environment for sequence data. In Bruijn FJD. (editor) Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches Hoboken, NJ: Wiley; 2011; pp. 399– 406 [Crossref]
-
11. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York, NY: Academic Press; 1969; pp. 21– 132 [Crossref]
-
12. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30: 1312– 1313 [CrossRef] [PubMed]
-
13. Felsenstein J. Phylip (Phylogeny Inference Package) Version 3.6 , Distributed by the author. Seattle, USA: Department of Genome Sciences, University of Washington; 2005
-
14. Chen YG, Liu ZX, Peng DJ, Zhang YQ, Wang YX et al. Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil. Antonie van Leeuwenhoek 2009; 96: 323– 329 [CrossRef] [PubMed]
-
15. Heyndrickx M, Lebbe L, Kersters K, Hoste B, De Wachter R et al. Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. Int J Syst Bacteriol 1999; 49: 1083– 1090 [CrossRef] [PubMed]
-
16. Lu J, Nogi Y, Takami H. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 2001; 205: 291– 297 [CrossRef] [PubMed]
-
17. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
-
18. Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196: 2210– 2215 [CrossRef] [PubMed]
-
19. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
-
20. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp. 607– 654
-
21. Cowan ST, Steel KJ. Bacterial Characters and Characterization, 2nd ed. Cambridge, UK: Cambridge University Press; 1974
-
22. Dong XZ, Cai MY. Determination of biochemical characteristics. In Dong XZ, Cai MY. (editors) Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001; pp. 370– 398
-
23. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42: 457– 469 [CrossRef]
-
24. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007; pp. 330– 393

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