1887

Abstract

A novel strain designated Aerobe-19 was isolated from a soil sample collected from a lawn located in Seoul National University in Korea. The cells were Gram-stain-positive, aerobic, and coccus-shaped. Colonies were circular with entire edges, convex, opaque and pale yellow. The strain grew at 15–30 ˚C (optimum, 30 ˚C), pH 5.0–7.0 (optimum, 6.0) and in the presence of 0–0.5 % (w/v) NaCl. Phylogenetically, the strain was found to be closely related to members of the genus and showed 16S rRNA gene sequence similarities of 96.4 and 96.3 % with NRRL B-24347 and KACC 16543, respectively. The major cellular fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The predominant menaquinone was MK-8 (H). The polar lipids profile revealed the presence of diphosphatidylglycerol, glycolipids, unknown amino-glycophospholipid, unknown phospholipid and unknown lipids. The peptidoglycan type was A1γ. The DNA G+C content of this stain was 73.9 mol%. On the basis of data presented, strain Aerobe-19 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Aerobe-19 (=KACC 18485 =KCTC 39705=NBRC 111450).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002231
2017-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/3960.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002231&mimeType=html&fmt=ahah

References

  1. Kageyama A, Takahashi Y, Seki T, Tomoda H, Omura S. Oryzihumus leptocrescens gen. nov., sp. nov. Int J Syst Evol Microbiol 2005; 55:2555–2559 [View Article][PubMed]
    [Google Scholar]
  2. Lim JM, Kim SJ, Hamada M, Ahn JH, Weon HY et al. Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus . Int J Syst Evol Microbiol 2014; 64:2395–2399 [View Article][PubMed]
    [Google Scholar]
  3. Kim DU, Ka JO. Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 2014; 64:1024–1029 [View Article][PubMed]
    [Google Scholar]
  4. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  5. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  6. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York, NY: Academic Press; 1969 pp. 21–132 [Crossref]
    [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  8. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  9. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  11. Breznak JA, Costilow RN. Physicochemical factors in growth. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR. (editors) Methods for General and Molecular Bacteriology, 3rd ed. Washington, DC: American Society For Microbiology; 2007 pp. 309–329
    [Google Scholar]
  12. Smibert R, Krieg N. Phenotypic characterization. In Gerhardt P, Murra RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  13. Ten LN, Xu JL, Jin FX, Im WT, Oh HM et al. Spirosoma panaciterrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 2009; 59:331–335 [View Article][PubMed]
    [Google Scholar]
  14. Schumann P. Peptidoglycan structure. Methods Microbiol 2011; 38:101–129 [Crossref]
    [Google Scholar]
  15. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002231
Loading
/content/journal/ijsem/10.1099/ijsem.0.002231
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error