@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002236, author = "Park, Sooyeon and Yoon, Sun Young and Park, Ji-Min and Yoon, Jung-Hoon", title = "Polaribacter insulae sp. nov., isolated from a tidal flat", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "4013-4019", doi = "https://doi.org/10.1099/ijsem.0.002236", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002236", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Polaribacter insulae", keywords = "novel species", keywords = "polyphasic taxonomy", keywords = "tidal flat", abstract = "A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-22T, was isolated from a tidal flat of Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-22T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OITF-22T fell within the clade comprising the type strains of Polaribacter species. Strain OITF-22T exhibited 16S rRNA gene sequence similarity values of 97.2–99.4 % to the type strains of Polaribacter vadi , P. haliotis , P. atrinae , P. dokdonensis , P. litorisediminis, P. reichenbachii , P. irgensii and P. marinaquae , and of 93.0–96.9 % to the type strains of the other Polaribacter species. Strain OITF-22T contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C15 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain OITF-22T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-22T was 32.3 mol% and its DNA–DNA relatedness values with the type strains of the eight phylogenetically most closely related Polaribacter species were 9–32 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain OITF-22T is separated from recognized species of the genus Polaribacter . On the basis of the data presented, strain OITF-22T is considered to represent a novel species of the genus Polaribacter , for which the name Polaribacter insulae sp. nov. is proposed. The type strain is OITF-22T (=KCTC 52658T=NBRC 112706T).", }