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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing Free

Abstract

Obligately alkaliphilic, indigo-reducing strains, designated Bps-1, Bps-2 and Bps-3, were isolated from an indigo fermentation liquor used for dyeing, which was produced from (composted indigo leaves) obtained from a craft centre in Data City, Hokkaido, Japan, by using medium containing cellulase-treated . The 16S rRNA gene sequence phylogeny suggested that Bps-1 has a distinctive position among the alkaliphilic species of the genus , with its closest neighbours being DSM 8715, DSM 26864 and DSM 485 (96.1, 95.8 and 95.5 % 16S rRNA gene sequence similarities, respectively). The 16S rRNA sequence of strain Bps-1 was identical to those of strains Bps-2 and Bps-3. Cells of the novel isolate were Gram-stain-positive and were facultatively anaerobic straight rods that were motile by means of a pair of flagella (subpolar and centre sides). Spherical endospores were formed in the terminal position. Strain Bps-1 grew between 18 and 40 °C with optimum growth at 33 °C. The isolate grew in the pH range 8‒11, with optimum growth at pH 9‒10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 40.3 %. The whole-cell fatty acid profile (>10 %) mainly consisted of anteiso-C, iso-C and C. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolates represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of this species is Bps-1 (JCM 31808=NCIMB 15080), with strains Bps-2 and Bps-3 representing additional strains of the species.

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  • Accepted:
  • Published Online:
Keyword(s): alkaliphilic , indigo fermentation liquor , indigo reduction and Paralkalibacillus indicireducens
© 2017 IUMS
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2017-10-01
2024-04-19
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Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing
Int J Syst Evol Microbiol 67, 4050 (2017); https://doi.org/10.1099/ijsem.0.002248
/content/journal/ijsem/10.1099/ijsem.0.002248
/content/journal/ijsem/10.1099/ijsem.0.002248
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