%0 Journal Article %A Lee, Hyosun %A Kim, Dong-Uk %A Lee, Suyeon %A Yun, Jungpyo %A Park, Sooyeon %A Yoon, Jung-Hoon %A Park, So Yoon %A Ka, Jong-Ok %T Sphingomonas carri sp. nov., isolated from a car air-conditioning system %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 10 %P 4069-4074 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002250 %K Sphingomonas carri %K novel species %K car evaporator core %K polyphasic taxonomy %I Microbiology Society, %X A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR0302T, was isolated from a car evaporator core collected in Korea. The cells were strictly aerobic, non-spore-forming and rod-shaped. The strain grew at 15–37 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Sphingomonas (97.04–91.22 % 16S rRNA gene sequence similarities) and showed the highest sequence similarity of 97.04 % to Sphingomonas kyeonggiensis THG-DT81T. It contained C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C14 : 0 2-OH as the predominant fatty acids and Q-10 as the major ubiquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The major polyamine was sym-homospermidine. The serine palmitoyl transferase gene (spt) was detected and sphingolipid synthesis was confirmed. The mean DNA G+C content of the strain was 67.8±0.5 mol%. DNA–DNA relatedness between strain PR0302T and closely related type strains of Sphingomonas species was less than 30 %. The low levels of DNA–DNA relatedness identified strain PR0302T as a member of a novel species in the genus Sphingomonas . Based on phenotypic, genotypic and chemotaxonomic data, strain PR0302T represents a novel species in the genus Sphingomonas , for which the name Sphingomonas carri sp. nov. is proposed. The type strain is PR0302T (=KACC 18487T=NBRC 111532T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002250