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- Volume 67, Issue 10
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f Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov
- Authors: Mahdi Moshtaghi Nikou1 , Mohaddaseh Ramezani1 , Sharareh Harirchi1 , Somayyeh Makzoom1 , Mohammad Ali Amoozegar2 , Seyed Abolhassan Shahzadeh Fazeli1,3 , Peter Schumann4 , Antonio Ventosa5
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- VIEW AFFILIATIONS
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1 1Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran 2 2Department of Microbiology, Extremophiles Laboratory, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 3 3Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran 4 4Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 5 5Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
- *Correspondence: Mohammad Ali Amoozegar [email protected]
- First Published Online: 18 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4221-4227, doi: 10.1099/ijsem.0.002286
- Subject: New taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov, Page 1 of 1
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A Gram-positive, halophilic actinobacterial strain Miq-12T was isolated from Meighan wetland in Iran. Strain Miq-12T was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12–25 % NaCl, at 30–50 °C and pH 5.5–10.5. The optimum NaCl, temperature and pH for growth were 15–20 %, 40 °C and 7.0–8.0, respectively. The cell wall of strain Miq-12T contained meso-diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. It synthesized cellular fatty acids of anteiso and iso-branched types, anteiso-C17 : 0, iso-C17:0, iso-C15:0, iso-C16 : 0. The major respiratory quinone was MK-9(H4). The G+C content of its genomic DNA was 72.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Miq-12T belongs to the family Pseudonocardiaceae , constituted a separate clade, and showed the closest phylogenetic similarity to Saccharopolyspora aidingensis TRM 46074T (96.99 %) and Saccharopolyspora ghardaiensis CCUG 63370T (96.92 %). On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, a novel genus and species of the family Pseudonocardiaceae , Salinifilum proteinilyticum gen. nov., sp. nov., are proposed. The type strain is Miq-12T (=IBRCM 11033T=LMG 28390T). We also propose that S. aidingensis and S. ghardaiensis should be transferred to this new genus and be named Salinifilum aidingensis comb. nov. and Salinifilum ghardaiensis comb. nov., respectively. The type strain of Salinifilum aidingensis comb. nov. is TRM 46074T (=CCTCCAA 2012014T=JCM 30185T) and the type strain of Salinifilum ghardaiensis comb. nov. is CCUG 63370T (=DSM 45606T=CECT 8304T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Miq-12T is KX024578.
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One supplementary figure is available with the online Supplementary Material.
- Keyword(s): extremely halophilic, actinobacteria, rare actinomycete, Meighan wetland
© 2017 IUMS | Published by the Microbiology Society
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