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f Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov

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  • Authors: Mahdi Moshtaghi Nikou1​ ,  Mohaddaseh Ramezani1​ ,  Sharareh Harirchi1​ ,  Somayyeh Makzoom1​ ,  Mohammad Ali Amoozegar2​ ,  Seyed Abolhassan Shahzadeh Fazeli1​,3​ ,  Peter Schumann4​ , Antonio Ventosa5​
    • VIEW AFFILIATIONS
    • 1​ 1​Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran 2​ 2​Department of Microbiology, Extremophiles Laboratory, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 3​ 3​Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran 4​ 4​Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 5​ 5​Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
  • *Correspondence: Mohammad Ali Amoozegar [email protected]
  • First Published Online: 18 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4221-4227, doi: 10.1099/ijsem.0.002286
  • Subject: New taxa - Actinobacteria
  • Received:
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Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov, Page 1 of 1

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  • A Gram-positive, halophilic actinobacterial strain Miq-12 was isolated from Meighan wetland in Iran. Strain Miq-12 was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12–25 % NaCl, at 30–50 °C and pH 5.5–10.5. The optimum NaCl, temperature and pH for growth were 15–20 %, 40 °C and 7.0–8.0, respectively. The cell wall of strain Miq-12 contained meso-diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. It synthesized cellular fatty acids of anteiso and iso-branched types, anteiso-C17 : 0, iso-C17:0, iso-C15:0, iso-C16 : 0. The major respiratory quinone was MK-9(H4). The G+C content of its genomic DNA was 72.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Miq-12 belongs to the family Pseudonocardiaceae , constituted a separate clade, and showed the closest phylogenetic similarity to Saccharopolyspora aidingensis TRM 46074 (96.99 %) and Saccharopolyspora ghardaiensis CCUG 63370 (96.92 %). On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, a novel genus and species of the family Pseudonocardiaceae , Salinifilum proteinilyticum gen. nov., sp. nov., are proposed. The type strain is Miq-12 (=IBRCM 11033=LMG 28390). We also propose that S. aidingensis and S. ghardaiensis should be transferred to this new genus and be named Salinifilum aidingensis comb. nov. and Salinifilum ghardaiensis comb. nov., respectively. The type strain of Salinifilum aidingensis comb. nov. is TRM 46074 (=CCTCCAA 2012014=JCM 30185) and the type strain of Salinifilum ghardaiensis comb. nov. is CCUG 63370 (=DSM 45606=CECT 8304).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Miq-12 is KX024578.

  • One supplementary figure is available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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