@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002293, author = "Martina, Pablo and Leguizamon, Mariana and Prieto, Claudia I. and Sousa, Silvia A. and Montanaro, Patricia and Draghi, Walter O. and Stämmler, Maren and Bettiol, Marisa and de Carvalho, Carla C. C. R. and Palau, Juliana and Figoli, Cecilia and Alvarez, Florencia and Benetti, Silvina and Lejona, Sergio and Vescina, Cecilia and Ferreras, Julián and Lasch, Peter and Lagares, Antonio and Zorreguieta, Angeles and Leitão, Jorge H. and Yantorno, Osvaldo M. and Bosch, Alejandra", title = "Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "14-20", doi = "https://doi.org/10.1099/ijsem.0.002293", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002293", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Burkholderia cepacia complex", keywords = "Burkholderia genus", keywords = "Burkholderia puraquae sp. nov", abstract = "Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3 % from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.", }