Paenibacillus arcticus sp. nov., isolated from Arctic soil

In-Tae Cha; Eui-Sang Cho; Yesol Yoo; Yoon Ji Seok; Inhye Park; Hee Seon Lim; Jung-Min Park; Seong Woon Roh; Young-Do Nam; Hak-Jong Choi; Yoo Kyung Lee; Myung-Ji Seo

doi:10.1099/ijsem.0.002299

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f Paenibacillus arcticus sp. nov., isolated from Arctic soil

Authors: In-Tae Cha^1,† , Eui-Sang Cho^2,† , Yesol Yoo³ , Yoon Ji Seok³ , Inhye Park³ , Hee Seon Lim³ , Jung-Min Park⁴ , Seong Woon Roh⁵ , Young-Do Nam⁶ , Hak-Jong Choi⁵ , Yoo Kyung Lee⁷ , Myung-Ji Seo^1,2
- VIEW AFFILIATIONS
- ¹ 1Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea ² 2Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon 22012, Republic of Korea ³ 3Department of Life Sciences, Graduate School of Incheon National University, Incheon 22012, Republic of Korea ⁴ 4Korean Culture Center of Microorganisms, Seoul 03641, Republic of Korea ⁵ 5Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea ⁶ 6Research Group of Gut Microbiome, Korea Food Research Institute, Seongnam 13539, Republic of Korea ⁷ 7Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea
*Correspondence: Myung-Ji Seo [email protected]
First Published Online: 18 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4385-4389, doi: 10.1099/ijsem.0.002299
Subject: New taxa - Firmicutes and Related Organisms

Received: 31/01/2017
Accepted: 15/08/2017
Cover date: 01/11/2017

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A Gram-positive, endospore-forming, strictly aerobic and rod-shaped bacterium, designated strain MME2_R6T, was isolated from Arctic soil, and it was identified by using a polyphasic taxonomic approach. This strain was psychrotolerant, growing at 4‒24 °C. 16S rRNA gene sequence analysis showed that strain MME2_R6T was closest to Paenibacillus swuensis DY6T, with 93.9 % similarity. However, in phylogenetic analysis based on the 16S rRNA gene sequence, strain MME2_R6T showed that it clustered with Paenibacillus contaminans CKOBP-6T and the sequencing similarity between the two species was 93.7 %. Its major cellular fatty acid was anteiso-C_15 : 0, like other Paenibacillus species. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant isoprenoid quinone was menaquinone-7. The diagnostic diamino acid in the cell wall was meso-diaminopimelic acid. The genomic DNA G+C content was 44.2 mol%. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, a novel species, Paenibacillus arcticus sp. nov., is proposed. The type strain is MME2_R6T (=JCM 30981T=PAMC 28731T).

†
These authors contributed equally to this work.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MME_R6T is KP900012.
Three supplementary figures are available with the online Supplementary Material.

Keyword(s): Arctic, polyphasic taxonomy, Paenibacillus arcticus

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