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f Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water

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  • Authors: Long Jin1​ ,  So-Ra Ko2​ ,  Chang Soo Lee3​ ,  Chi-Yong Ahn2​ ,  Hee-Mock Oh2​ , Hyung-Gwan Lee2​
    • VIEW AFFILIATIONS
    • 1​ 1​College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210-037, PR China 2​ 2​Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea 3​ 3​Culture Collection Team, Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea
  • *Correspondence: Hee-Mock Oh [email protected], Hyung-Gwan Lee [email protected]
  • First Published Online: 28 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4443-4448, doi: 10.1099/ijsem.0.002311
  • Subject: New taxa - Proteobacteria
  • Received:
  • Accepted:
  • Cover date:
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Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water, Page 1 of 1

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  • A novel Gram-negative bacterium strain, DRW22-8, was isolated from fresh water taken at a depth of 22 m at Daechung Reservoir, Republic of Korea. The cells of strain DRW22-8 were aerobic and motile with a single polar flagellum or non-motile (stalked), and formed creamy-white colonies on R2A agar. The phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order Rhodobacterales , showing similarity values under 91.8 % with its closest phylogenetic neighbours, Hirschia litorea , Hirschia baltica and Hirschia maritima . The chemotaxonomic results showed Q-10 as the predominant respiratory ubiquinone, three unidentified glycolipids, an unidentified lipid and phosphatidylglycerol as the major polar lipids, and C16 : 0, 11-methyl C18 : 1, C18 : 1  ω7c and/or C18 : 1  ω6c as the major fatty acids. The DNA G+C content was 64.4 mol%. The combined genotypic and phenotypic data showed that strain DRW22-8 could be distinguished from all genera within the family Hyphomonadaceae and represented a novel genus, Asprobacter gen. nov., with the name Asprobacter aquaticus sp. nov., in the family Hyphomonadaceae . The type strain is DRW22-8 (=KCTC 42356=JCM 30469).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DRW22-8 is KF056993.

  • One supplementary figure is available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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