Bacillus solisilvae sp. nov., isolated from forest soil
Pan, Tong and He, Hairong and Wang, Xiaochong and Shen, Yibo and Zhao, Junwei and Yan, Kai and Wang, Xiangjing and Liu, Chongxi and Zhang, Ji and Xiang, Wensheng,, 67, 4449-4455 (2017),
doi = https://doi.org/10.1099/ijsem.0.002312,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterial strain, NEAU-cbsb5T, was isolated from forest soil from Changbai Mountain, Heilongjiang Province, China. The isolate grew at 15–40 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-cbsb5T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus acidiceler DSM 18954T (99.1 % similarity) and Bacillus luciferensis JCM 12212T (99.0 %). 16S rRNA gene sequence similarity to sequences of the type strains of other Bacillus species was less than 96.0 %. Average nucleotide identity (ANI) values between NEAU-cbsb5T and its most closely related species were 78.72–84.75 % by ANIm, ANIb and OrthoANIu analysis. The in silico DNA–DNA hybridization values between strain NEAU-cbsb5T and its close relatives B. acidiceler DSM 18954T and B. luciferensis JCM 12212T were both 23.80 %, again indicating they belong to different taxa. The major cellular fatty acids of NEAU-cbsb5T were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unknown aminophospholipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 33.0 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain NEAU-cbsb5T was classified as a representative of a novel species in the genus Bacillus , for which the name Bacillus solisilvae sp. nov. is proposed. The type strain is NEAU-cbsb5T (=CGMCC 1.14993T=DSM 100485T).,
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