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Volume 67, Issue 11

sp. nov., an extremely halophilic archaeon isolated from a solar saltern Free

Abstract

An extremely halophilic archaeal strain SP28 was isolated from the Gomso solar saltern, Republic of Korea. Cells of the new strain SP28 were pleomorphic and , and produced red-pigmented colonies. These grew in medium with 2.5–4.5 M NaCl (optimum 3.1 M) and 0.05–0.5 M MgCl (optimum 0.1 M), at 25–50 °C (optimum 37 °C) and at a pH of 6.5–8.5 (optimum pH 8.0). Mg was required for growth. A concentration of at least 2 M NaCl was required to prevent cell lysis. Polar lipids included phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one glycolipid chromatographically identical to sulfated mannosyl glucosyl diether. 16S rRNA and ′ gene sequence analyses showed that strain SP28 is closely related to R35 (97.3 and 94.1 %, 16S rRNA and gene sequence similarity, respectively), GX21 (97.0 and 92.1 %), YGH66 (96.0 and 91.9 %), RO5-8 (95.9 and 90.9 %), TBN37 (95.6 and 90.3 %) and RE-101 (95.3 and 89.8 %). The DNA G+C content of the novel strain SP28 was 66.2 mol%, which is slightly higher than that of GX21 (65.8 mol%) and R35 (66.0 mol%). DNA–DNA hybridization values between R35 and strain SP28 and between GX21 and strain SP28 were about 24.8 and 20.7 %, respectively. We conclude that strain SP28 represents a novel species of the genus and propose the name sp. nov. The type strain is SP28 (=JCM 31424=KCCM 43210).

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  • Accepted:
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Keyword(s): halophilic archaeon , Haloplanus , Haloplanus salinarum and solar saltern
© 2017 IUMS
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2017-11-01
2024-04-19
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Haloplanus salinarum sp. nov., an extremely halophilic archaeon isolated from a solar saltern
Int J Syst Evol Microbiol 67, 4456 (2017); https://doi.org/10.1099/ijsem.0.002313
/content/journal/ijsem/10.1099/ijsem.0.002313
/content/journal/ijsem/10.1099/ijsem.0.002313
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