Ramlibacter monticola sp. nov., isolated from forest soil Chaudhary, Dhiraj Kumar and Kim, Jaisoo,, 67, 4468-4474 (2017), doi = https://doi.org/10.1099/ijsem.0.002314, publicationName = Microbiology Society, issn = 1466-5026, abstract= During a study of bacterial diversity in forest soil, strain G-3-2T, a Gram-stain-negative, light brown-coloured, non-motile, rod- or coccoid-shaped bacterium, was isolated. It was able to grow at 15–37 °C, at pH 5.5–10.0 and at 0–0.5 % (w/v) NaCl concentration. The strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain G-3-2T belongs to the genus Ramlibacter and is closely related to Ramlibacter ginsenosidimutans BXN5-27T (98.69 % sequence similarity), Ramlibacter henchirensis TMB834T (96.98 %), Ramlibacter tataouinensis TTB310T (96.49 %) and Ramlibacter solisilvae 5-10T (96.42 %). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids of strain G-3–2T were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1 ω6c), C17 : 0 cyclo, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C10 : 0 3-OH. The genomic DNA G+C content of this novel strain was 68.9 mol%. The DNA–DNA relatedness between strain G-3-2T and R. ginsenosidimutans BXN5-27T was 44.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain G-3-2T represents a novel species of the genus Ramlibacter , for which the name Ramlibacter monticola sp. nov. is proposed. The type strain is G-3-2T (=KEMB 9005-573T=KACC 19175T=JCM 31918T)., language=, type=