@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002326, author = "Yang, Seung-Jo and Kim, Byung-Yong and Chun, Jongsik", title = "Rejection of reclassification of Lactobacillus kimchii and Lactobacillus bobalius as later subjective synonyms of Lactobacillus paralimentarius using comparative genomics", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "11", pages = "4515-4517", doi = "https://doi.org/10.1099/ijsem.0.002326", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002326", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "reclassification", keywords = "genome sequencing", keywords = "average nucleotide identity", keywords = "lactobacillus", abstract = " Lactobacillus bobalius , Lactobacillus kimchii and Lactobacillus paralimentarius belong to the genus Lactobacillus and show close phylogenetic relationships. In a previous study, L. bobalius and L. kimchii were proposed to be reclassified as later heterotypic synonyms of L. paralimentarius using high 16S rRNA gene sequence similarities (≥99.5 %) and DNA–DNA hybridization values (≥82 %). We determined high quality whole genome assemblies of the type strains of L. bobalius and L. kimchii , which were then compared with that of L. paralimentarius . Average nucleotide identity values among three genomes ranged from 91.4 to 92.3 % which are clearly below 95~96 %, the generally recognized cutoff value for bacterial species boundaries. On the basis of comparative genomic evidence, L. bobalius , L. kimchii , and L. paralimentarius should stand as separate species in the genus Lactobacillus . We therefore suggest rejecting the previous proposal to combine these three species into a single species.", }